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Adipokinetic hormone-induced antioxidant response in Spodoptera littoralis
J. Večeřa, N. Krishnan, A. Mithöfer, H. Vogel, D. Kodrík,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- antioxidancia metabolismus MeSH
- dieta MeSH
- glutathion metabolismus MeSH
- glutathiontransferasa metabolismus MeSH
- hmyzí hormony farmakologie MeSH
- hmyzí proteiny genetika metabolismus MeSH
- katalasa genetika metabolismus MeSH
- kyselina pyrrolidonkarboxylová analogy a deriváty farmakologie MeSH
- larva účinky léků genetika metabolismus MeSH
- Manduca chemie MeSH
- oligopeptidy farmakologie MeSH
- oxidační stres účinky léků MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- regulace genové exprese enzymů účinky léků MeSH
- Spodoptera účinky léků genetika metabolismus MeSH
- superoxiddismutasa genetika metabolismus MeSH
- taniny aplikace a dávkování MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The antioxidative potential of the Manduca sexta adipokinetic hormone (Manse-AKH) in the last instar larvae of Spodoptera littoralis (Noctuidae, Lepidoptera) was demonstrated after exposure to oxidative stress (OS) elicited by feeding on artificial diet containing tannic acid (TA). Determination of protein carbonyls (PCs) and reduced glutathione (GSH) levels, monitoring of activity of antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferases (GSTs), as well as measuring of the mRNA expression of CAT and SOD were used as markers of the OS. Injection of the Manse-AKH (5 pmol per individual) reversed the OS status by mitigation of PCs formation and by stimulation of glutathione-S-transferases (GSTs) activity. The CAT and SOD mRNA expression was significantly suppressed after the Manse-AKH injection while activity of these enzymes was not affected. These results indicate that diminishing of OS after the AKH injection might be a result of activation of specific enzymatic pathway possibly at the post-translational level rather than a direct effect on regulation of antioxidant marker genes at the transcriptional level.
Citace poskytuje Crossref.org
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- $a The antioxidative potential of the Manduca sexta adipokinetic hormone (Manse-AKH) in the last instar larvae of Spodoptera littoralis (Noctuidae, Lepidoptera) was demonstrated after exposure to oxidative stress (OS) elicited by feeding on artificial diet containing tannic acid (TA). Determination of protein carbonyls (PCs) and reduced glutathione (GSH) levels, monitoring of activity of antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferases (GSTs), as well as measuring of the mRNA expression of CAT and SOD were used as markers of the OS. Injection of the Manse-AKH (5 pmol per individual) reversed the OS status by mitigation of PCs formation and by stimulation of glutathione-S-transferases (GSTs) activity. The CAT and SOD mRNA expression was significantly suppressed after the Manse-AKH injection while activity of these enzymes was not affected. These results indicate that diminishing of OS after the AKH injection might be a result of activation of specific enzymatic pathway possibly at the post-translational level rather than a direct effect on regulation of antioxidant marker genes at the transcriptional level.
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