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Biodiversity of avian trypanosomes
L. Zídková, I. Cepicka, J. Szabová, M. Svobodová,
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biodiversity MeSH
- DNA Primers MeSH
- Phylogeny MeSH
- DNA, Protozoan genetics MeSH
- Birds parasitology MeSH
- RNA, Ribosomal genetics MeSH
- Sequence Analysis, DNA MeSH
- Random Amplified Polymorphic DNA Technique methods MeSH
- Microscopy, Electron, Transmission MeSH
- Trypanosoma classification genetics isolation & purification MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
We have studied the biodiversity of trypanosomes from birds and bloodsucking Diptera on a large number of isolates. We used two molecular approaches, random amplification of polymorphic DNA (RAPD) method, and sequence analysis of the small subunit ribosomal RNA (SSU rRNA) gene. RAPD method divided the isolates into 11 separate lineages. Phylogenetic analysis of the SSU rRNA gene was congruent with the RAPD. Morphometric analysis of kinetoplast width and cell length was in agreement with molecular data. Avian trypanosomes appeared polyphyletic on SSU rDNA tree; thus, they do not represent a taxonomic group. We propose that all lineages recovered by SSU analysis probably represent distinct species of avian trypanosomes. We discuss possible transmission ways and geographical distribution of new avian trypanosome lineages. Finally, we recommend methods that should be used for species determination of avian trypanosomes.
References provided by Crossref.org
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