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Biodiversity of avian trypanosomes
L. Zídková, I. Cepicka, J. Szabová, M. Svobodová,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- biodiverzita MeSH
- DNA primery MeSH
- fylogeneze MeSH
- protozoální DNA genetika MeSH
- ptáci parazitologie MeSH
- RNA ribozomální genetika MeSH
- sekvenční analýza DNA MeSH
- technika náhodné amplifikace polymorfní DNA metody MeSH
- transmisní elektronová mikroskopie MeSH
- Trypanosoma klasifikace genetika izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
We have studied the biodiversity of trypanosomes from birds and bloodsucking Diptera on a large number of isolates. We used two molecular approaches, random amplification of polymorphic DNA (RAPD) method, and sequence analysis of the small subunit ribosomal RNA (SSU rRNA) gene. RAPD method divided the isolates into 11 separate lineages. Phylogenetic analysis of the SSU rRNA gene was congruent with the RAPD. Morphometric analysis of kinetoplast width and cell length was in agreement with molecular data. Avian trypanosomes appeared polyphyletic on SSU rDNA tree; thus, they do not represent a taxonomic group. We propose that all lineages recovered by SSU analysis probably represent distinct species of avian trypanosomes. We discuss possible transmission ways and geographical distribution of new avian trypanosome lineages. Finally, we recommend methods that should be used for species determination of avian trypanosomes.
Citace poskytuje Crossref.org
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