-
Something wrong with this record ?
Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by addition of follicular fluid
L. Bruckova, T. Soukup, B. Visek, J. Moos, M. Moosova, J. Pavelkova, K. Rezabek, L. Kucerova, S. Micuda, E. Brcakova, J. Mokry
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 2008 to 1 year ago
PubMed Central
from 1997 to 1 year ago
Europe PubMed Central
from 1997 to 1 year ago
ProQuest Central
from 1999-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2011-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 1999-01-01 to 1 year ago
Public Health Database (ProQuest)
from 1999-01-01 to 1 year ago
- MeSH
- Cell Culture Techniques MeSH
- Time Factors MeSH
- Phenotype MeSH
- Granulosa Cells cytology MeSH
- Follicular Fluid MeSH
- Karyotyping MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Cell Proliferation MeSH
- Flow Cytometry MeSH
- Telomere MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
PURPOSE: The aim of this study was to develop and optimize a strategy for long-term cultivation of luteinizing human granulosa cells (GCs). METHODS: GCs were cultivated in DMEM/F12 medium supplemented with 2% fetal calf serum. In vitro proliferation of GCs was supported by follicular fluid as well as FSH and growth factors. RESULTS: The cultured GCs were maintained for 45 days with a doubling time of 159 ± 24 h. GCs initiated by the addition of follicular fluid and cultivated under low serum conditions reached 10 ± 0.7 population doublings. GCs maintain the typical phenotypic expression and the telomere length according to specific culture conditions. CONCLUSION: Our present study has demonstrated that GCs can be maintained in vitro for at least 45 days and this cell model can be beneficial when studying hormonal regulation associated with follicular maturation and preparation of oocytes for fertilization.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12024393
- 003
- CZ-PrNML
- 005
- 20231116134252.0
- 007
- ta
- 008
- 120815s2011 ne f 000 0#eng||
- 009
- AR
- 024 7_
- $a 10.1007/s10815-011-9617-6 $2 doi
- 035 __
- $a (PubMed)21822582
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Brůčková, Lenka. $7 _BN007269 $u Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
- 245 10
- $a Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by addition of follicular fluid / $c L. Bruckova, T. Soukup, B. Visek, J. Moos, M. Moosova, J. Pavelkova, K. Rezabek, L. Kucerova, S. Micuda, E. Brcakova, J. Mokry
- 520 9_
- $a PURPOSE: The aim of this study was to develop and optimize a strategy for long-term cultivation of luteinizing human granulosa cells (GCs). METHODS: GCs were cultivated in DMEM/F12 medium supplemented with 2% fetal calf serum. In vitro proliferation of GCs was supported by follicular fluid as well as FSH and growth factors. RESULTS: The cultured GCs were maintained for 45 days with a doubling time of 159 ± 24 h. GCs initiated by the addition of follicular fluid and cultivated under low serum conditions reached 10 ± 0.7 population doublings. GCs maintain the typical phenotypic expression and the telomere length according to specific culture conditions. CONCLUSION: Our present study has demonstrated that GCs can be maintained in vitro for at least 45 days and this cell model can be beneficial when studying hormonal regulation associated with follicular maturation and preparation of oocytes for fertilization.
- 650 _2
- $a buněčné kultury $7 D018929
- 650 _2
- $a proliferace buněk $7 D049109
- 650 _2
- $a kultivované buňky $7 D002478
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a průtoková cytometrie $7 D005434
- 650 _2
- $a folikulární tekutina $7 D015571
- 650 _2
- $a folikulární buňky $x cytologie $7 D006107
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a karyotypizace $7 D007621
- 650 _2
- $a fenotyp $7 D010641
- 650 _2
- $a telomery $7 D016615
- 650 _2
- $a časové faktory $7 D013997
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Soukup, Tomáš, $d 1980- $7 xx0036274
- 700 1_
- $a Víšek, Benjamín $7 xx0089713
- 700 1_
- $a Moos, Jiří $7 xx0093748
- 700 1_
- $a Moosová, Martina $7 xx0310038
- 700 1_
- $a Pavelková, Jana
- 700 1_
- $a Rezabek, Karel
- 700 1_
- $a Kučerová, Lenka $7 xx0142635
- 700 1_
- $a Mičuda, Stanislav, $d 1972- $7 jn20010309083
- 700 1#
- $a Doleželová, Eva. $7 xx0179807
- 700 1_
- $a Mokrý, Jaroslav, $d 1964- $7 jn20000620235
- 773 0_
- $w MED00002533 $t Journal of assisted reproduction and genetics $x 1573-7330 $g Roč. 28, č. 10 (2011), s. 939-50
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/21822582 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y m $z 0
- 990 __
- $a 20120815 $b ABA008
- 991 __
- $a 20231116134246 $b ABA008
- 999 __
- $a ok $b bmc $g 946541 $s 781721
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2011 $b 28 $c 10 $d 939-50 $e 20110806 $i 1573-7330 $m Journal of assisted reproduction and genetics $n J Assist Reprod Genet $x MED00002533
- LZP __
- $a Pubmed-20120815/12/02
