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Voltammetric detection of damage to DNA caused by nitro derivatives of fluorene using an electrochemical DNA biosensor
V. Vyskocil, J. Labuda, J. Barek
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- biosenzitivní techniky MeSH
- DNA analýza chemie MeSH
- elektrochemie MeSH
- fluoreny farmakologie MeSH
- indikátory a reagencie analýza chemie MeSH
- poškození DNA genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
An electrochemical DNA biosensor based on the screen printed carbon paste electrode (SPCPE) with an immobilized layer of calf thymus double-stranded DNA has been used for in vitro investigation of the interaction between genotoxic nitro derivatives of fluorene (namely 2-nitrofluorene and 2,7-dinitrofluorene) and DNA. Two types of DNA damage have been detected at the DNA/SPCPE biosensor: first, that caused by direct association of the nitrofluorenes, for which an intercalation association has been found using the known DNA intercalators [Cu(phen)(2)](2+) and [Co(phen)(3)](3+) as competing agents, and, second, that caused by short-lived radicals generated by electrochemical reduction of the nitro group (observable under specific conditions only).
Citace poskytuje Crossref.org
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