-
Something wrong with this record ?
Alterations in the vascular architecture of the dorsal root ganglia in a rat neuropathic pain model
L. Kubíček, R. Kopáčik, I. Klusáková, P. Dubový
Language English Country Germany
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Blood Vessels innervation physiopathology MeSH
- Functional Laterality MeSH
- Hyperalgesia physiopathology MeSH
- Rats MeSH
- Peripheral Nervous System Diseases physiopathology MeSH
- Sensory Receptor Cells cytology pathology physiology MeSH
- Sciatic Nerve physiopathology MeSH
- Neurons physiology MeSH
- Image Processing, Computer-Assisted MeSH
- Rats, Wistar MeSH
- Ganglia, Spinal anatomy & histology blood supply MeSH
- Wallerian Degeneration physiopathology MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
An alteration in the structural arrangement of blood vessels identified by RECA immunohistochemistry was studied in a rat L4 dorsal root ganglia (L4-DRG) neuropathic pain model. We compared a three-dimensional (3-D) reconstruction of the vascular architecture surrounding bodies of the primary sensory neurons in the L4-DRG of naïve rats with that of rats that had surgically undergone unilateral sciatic nerve ligature. Rhodamine-conjugated dextran (Fluoro-Ruby) was used for retrograde labelling of neurons, the axons of which had been injured by nerve ligature. In contrast to DRG from naïve rats and contralateral DRG from operated rats, an increased proportion of RECA+ vascular area and the appearance of nest-like arrangements of blood vessels around neuronal bodies with injured axons were observed in L4-DRG ipsilateral to the sciatic nerve ligature. Fractal analysis confirmed a higher degree of vascular branching, irregularity, and tortuosity in L4-DRG related with sciatic nerve injury. The results suggest that nerve injury induces changes in vascular architecture in associated DRG.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12025929
- 003
- CZ-PrNML
- 005
- 20130317160201.0
- 007
- ta
- 008
- 120817e20100201gw f 000 0#eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.aanat.2010.01.005 $2 doi
- 035 __
- $a (PubMed)20149608
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Kubíček, Luboš $u Department of Anatomy, Division of Neuroanatomy, Faculty of Medicine, Masaryk University, Kamenice 3, CZ-625 00 Brno, Czech Republic.
- 245 10
- $a Alterations in the vascular architecture of the dorsal root ganglia in a rat neuropathic pain model / $c L. Kubíček, R. Kopáčik, I. Klusáková, P. Dubový
- 520 9_
- $a An alteration in the structural arrangement of blood vessels identified by RECA immunohistochemistry was studied in a rat L4 dorsal root ganglia (L4-DRG) neuropathic pain model. We compared a three-dimensional (3-D) reconstruction of the vascular architecture surrounding bodies of the primary sensory neurons in the L4-DRG of naïve rats with that of rats that had surgically undergone unilateral sciatic nerve ligature. Rhodamine-conjugated dextran (Fluoro-Ruby) was used for retrograde labelling of neurons, the axons of which had been injured by nerve ligature. In contrast to DRG from naïve rats and contralateral DRG from operated rats, an increased proportion of RECA+ vascular area and the appearance of nest-like arrangements of blood vessels around neuronal bodies with injured axons were observed in L4-DRG ipsilateral to the sciatic nerve ligature. Fractal analysis confirmed a higher degree of vascular branching, irregularity, and tortuosity in L4-DRG related with sciatic nerve injury. The results suggest that nerve injury induces changes in vascular architecture in associated DRG.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a cévy $x inervace $x patofyziologie $7 D001808
- 650 _2
- $a funkční lateralita $7 D007839
- 650 _2
- $a spinální ganglia $x anatomie a histologie $x krevní zásobení $7 D005727
- 650 _2
- $a hyperalgezie $x patofyziologie $7 D006930
- 650 _2
- $a počítačové zpracování obrazu $7 D007091
- 650 _2
- $a neurony $x fyziologie $7 D009474
- 650 _2
- $a nemoci periferního nervového systému $x patofyziologie $7 D010523
- 650 _2
- $a krysa rodu Rattus $7 D051381
- 650 _2
- $a potkani Wistar $7 D017208
- 650 _2
- $a nervus ischiadicus $x patofyziologie $7 D012584
- 650 _2
- $a nervové receptory $x cytologie $x patologie $x fyziologie $7 D011984
- 650 _2
- $a Wallerova degenerace $x patofyziologie $7 D014855
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Kopáčik, Roman
- 700 1_
- $a Klusáková, Ilona, $d 1956- $7 xx0020082
- 700 1_
- $a Dubový, Petr, $d 1952- $7 xx0037109
- 773 0_
- $w MED00000417 $t Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft $x 1618-0402 $g Roč. 192, č. 2 (20100201), s. 101-106
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/20149608 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y m
- 990 __
- $a 20120817 $b ABA008
- 991 __
- $a 20130317160433 $b ABA008
- 999 __
- $a ok $b bmc $g 947971 $s 783275
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2010 $b 192 $c 2 $d 101-106 $e 20100201 $i 1618-0402 $m Annals of anatomy $n Ann Anat $x MED00000417
- LZP __
- $a Pubmed-20120817/10/03
