-
Something wrong with this record ?
An acellular assay to assess the genotoxicity of complex mixtures of organic pollutants bound on size segregated aerosol. Part I: DNA adducts
J. Topinka, J. Hovorka, A. Milcova, J. Schmuczerova, J. Krouzek, P. Rossner, RJ. Sram,
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- DNA Adducts analysis MeSH
- Aerosols MeSH
- DNA drug effects MeSH
- Liver drug effects MeSH
- Rats MeSH
- Air Pollutants toxicity MeSH
- Statistics, Nonparametric MeSH
- Particulate Matter toxicity MeSH
- Polycyclic Aromatic Hydrocarbons chemistry toxicity MeSH
- DNA Damage drug effects MeSH
- Cattle MeSH
- Mutagenicity Tests methods MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
An acellular assay consisting of calf thymus DNA with/without rat liver microsomal S9 fraction was used to study the genotoxicity of complex mixtures of organic air pollutants bound to size segregated aerosols by means of DNA adduct analysis. We compared the genotoxicity of the organic extracts (EOMs) from three size fractions of aerosol ranging from 0.17μm to 10μm that were collected by high volume cascade impactors in four localities of the Czech Republic differing in the extent of the environmental pollution: (1) small village in proximity of a strip mine, (2) highway, (3) city center of Prague and (4) background station. The total DNA adduct levels induced by 100μg/ml of EOMs were analyzed by (32)P-postlabelling analysis with a nuclease P1 method for adduct enrichment. The main finding of the study was most of the observed genotoxicity was connected with a fine particulate matter fraction (<1μm). The concentrations of carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) in EOMs indicate that fine fractions (0.5-1μm) bound the highest amount of c-PAHs in all aerosol sampling sites, which might be related to the higher specific surface of this fraction as compared with a course fraction (1-10μm) and higher mass as compared with a condensational fraction (0.17-0.5μm). As for aerosol mass, both fine and condensational fractions are effective carriers of c-PAHs. Similarly, the DNA adduct levels per m(3) of air were highest for the fine fraction, while the condensational fraction (strip mine site and city center) revealed the highest DNA adduct levels in cases where aerosol mass is taken into consideration. A strong correlation was found between the c-PAHs and DNA adduct levels induced by EOMs in all the localities and for various size fractions (R(2)=0.98, p<0.001). It may be concluded that the analysis of total DNA adducts induced in an acellular assay with/without metabolic activation represents a relatively simple method to assess the genotoxic potential of various complex mixtures.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12026280
- 003
- CZ-PrNML
- 005
- 20121210101355.0
- 007
- ta
- 008
- 120817e20100630ne f 000 0#eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.toxlet.2010.06.016 $2 doi
- 035 __
- $a (PubMed)20600709
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Topinka, Jan $u Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v.v.i., Prague, Czech Republic. jtopinka@biomed.cas.cz
- 245 13
- $a An acellular assay to assess the genotoxicity of complex mixtures of organic pollutants bound on size segregated aerosol. Part I: DNA adducts / $c J. Topinka, J. Hovorka, A. Milcova, J. Schmuczerova, J. Krouzek, P. Rossner, RJ. Sram,
- 520 9_
- $a An acellular assay consisting of calf thymus DNA with/without rat liver microsomal S9 fraction was used to study the genotoxicity of complex mixtures of organic air pollutants bound to size segregated aerosols by means of DNA adduct analysis. We compared the genotoxicity of the organic extracts (EOMs) from three size fractions of aerosol ranging from 0.17μm to 10μm that were collected by high volume cascade impactors in four localities of the Czech Republic differing in the extent of the environmental pollution: (1) small village in proximity of a strip mine, (2) highway, (3) city center of Prague and (4) background station. The total DNA adduct levels induced by 100μg/ml of EOMs were analyzed by (32)P-postlabelling analysis with a nuclease P1 method for adduct enrichment. The main finding of the study was most of the observed genotoxicity was connected with a fine particulate matter fraction (<1μm). The concentrations of carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) in EOMs indicate that fine fractions (0.5-1μm) bound the highest amount of c-PAHs in all aerosol sampling sites, which might be related to the higher specific surface of this fraction as compared with a course fraction (1-10μm) and higher mass as compared with a condensational fraction (0.17-0.5μm). As for aerosol mass, both fine and condensational fractions are effective carriers of c-PAHs. Similarly, the DNA adduct levels per m(3) of air were highest for the fine fraction, while the condensational fraction (strip mine site and city center) revealed the highest DNA adduct levels in cases where aerosol mass is taken into consideration. A strong correlation was found between the c-PAHs and DNA adduct levels induced by EOMs in all the localities and for various size fractions (R(2)=0.98, p<0.001). It may be concluded that the analysis of total DNA adducts induced in an acellular assay with/without metabolic activation represents a relatively simple method to assess the genotoxic potential of various complex mixtures.
- 650 _2
- $a aerosoly $7 D000336
- 650 _2
- $a látky znečišťující vzduch $x toxicita $7 D000393
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a skot $7 D002417
- 650 _2
- $a DNA $x účinky léků $7 D004247
- 650 _2
- $a adukty DNA $x analýza $7 D018736
- 650 _2
- $a poškození DNA $x účinky léků $7 D004249
- 650 _2
- $a játra $x účinky léků $7 D008099
- 650 _2
- $a testy genotoxicity $x metody $7 D009152
- 650 _2
- $a pevné částice $x toxicita $7 D052638
- 650 _2
- $a polycyklické aromatické uhlovodíky $x chemie $x toxicita $7 D011084
- 650 _2
- $a krysa rodu Rattus $7 D051381
- 650 _2
- $a neparametrická statistika $7 D018709
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Hovorka, Jan
- 700 1_
- $a Milcova, Alena
- 700 1_
- $a Schmuczerova, Jana
- 700 1_
- $a Krouzek, Jiri
- 700 1_
- $a Rossner, Pavel
- 700 1_
- $a Sram, Radim J
- 773 0_
- $w MED00004537 $t Toxicology letters $x 1879-3169 $g Roč. 198, č. 3 (20100630), s. 304-11
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/20600709 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y m
- 990 __
- $a 20120817 $b ABA008
- 991 __
- $a 20121210101432 $b ABA008
- 999 __
- $a ok $b bmc $g 948322 $s 783626
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2010 $b 198 $c 3 $d 304-11 $e 20100630 $i 1879-3169 $m Toxicology letters $n Toxicol Lett $x MED00004537
- LZP __
- $a Pubmed-20120817/10/04
