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Testate amoebae examined by confocal and two-photon microscopy: implications for taxonomy and ecophysiology
Z. Burdíková, M. Capek, P. Ostašov, J. Machač, R. Pelc, E. A. Mitchell, L. Kubínová
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
ProQuest Central
from 2002-02-01 to 2022-12-31
Nursing & Allied Health Database (ProQuest)
from 2002-02-01 to 2022-12-31
Health & Medicine (ProQuest)
from 2002-02-01 to 2022-12-31
- MeSH
- Amoebozoa classification ultrastructure MeSH
- Microscopy methods MeSH
- Imaging, Three-Dimensional MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Testate amoebae (TA) are a group of free-living protozoa, important in ecology and paleoecology. Testate amoebae taxonomy is mainly based on the morphological features of the shell, as examined by means of light microscopy or (environmental) scanning electron microscopy (SEM/ESEM). We explored the potential applications of confocal laser scanning microscopy (CLSM), two photon excitation microscopy (TPEM), phase contrast, differential interference contrast (DIC Nomarski), and polarization microscopy to visualize TA shells and inner structures of living cells, which is not possible by SEM or environmental SEM. Images captured by CLSM and TPEM were utilized to create three-dimensional (3D) visualizations and to evaluate biovolume inside the shell by stereological methods, to assess the function of TA in ecosystems. This approach broadens the understanding of TA cell and shell morphology, and inner structures including organelles and endosymbionts, with potential implications in taxonomy and ecophysiology.
Laboratory of Soil Biology University of Neuchâtel Rue Emile Argand 11 CH 2000 Neuchâtel Switzerland
References provided by Crossref.org
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