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In vivo kinetics of U4/U6·U5 tri-snRNP formation in Cajal bodies

I. Novotný, M. Blažíková, D. Staněk, P. Herman, J. Malinsky

. 2011 ; 22 (4) : 513-523.

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc12027190
E-zdroje Online Plný text

NLK Free Medical Journals od 1992 do Před 2 měsíci
PubMed Central od 1992 do Před 2 měsíci
Europe PubMed Central od 1992 do Před 2 měsíci
Open Access Digital Library od 1989-11-01
Open Access Digital Library od 1997-01-01

Odkazy

PubMed 21177826
DOI 10.1091/mbc.e10-07-0560
Knihovny.cz E-zdroje

The U4/U6·U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP) is an essential pre-mRNA splicing factor, which is assembled in a stepwise manner before each round of splicing. It was previously shown that the tri-snRNP is formed in Cajal bodies (CBs), but little is known about the dynamics of this process. Here we created a mathematical model of tri-snRNP assembly in CBs and used it to fit kinetics of individual snRNPs monitored by fluorescence recovery after photobleaching. A global fitting of all kinetic data determined key reaction constants of tri-snRNP assembly. Our model predicts that the rates of di-snRNP and tri-snRNP assemblies are similar and that ∼230 tri-snRNPs are assembled in one CB per minute. Our analysis further indicates that tri-snRNP assembly is approximately 10-fold faster in CBs than in the surrounding nucleoplasm, which is fully consistent with the importance of CBs for snRNP formation in rapidly developing biological systems. Finally, the model predicted binding between SART3 and a CB component. We tested this prediction by Förster resonance energy transfer and revealed an interaction between SART3 and coilin in CBs.

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