-
Something wrong with this record ?
KPC-2-producing Klebsiella pneumoniae isolated from a Czech patient previously hospitalized in Greece and in vivo selection of colistin resistance
J. Hrabák, J. Niemczyková, E. Chudáčková, M. Fridrichová, V. Studentová, D. Cervená, P. Urbášková, H. Zemličková
Language English Country Czech Republic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NS9717
MZ0
CEP Register
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Drug Resistance, Bacterial drug effects genetics MeSH
- beta-Lactamases biosynthesis genetics MeSH
- Hospitalization MeSH
- Klebsiella pneumoniae drug effects enzymology genetics isolation & purification MeSH
- Colistin pharmacology MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Porins genetics MeSH
- Gene Expression Regulation, Bacterial MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Greece MeSH
Carbapenemase-producing Gram-negative bacteria peak clinical interest due to their ability to hydrolyze most β-lactams, including carbapenems; moreover, their genes spread through bacterial populations by horizontal transfer. Bacteria with acquired carbapenemase have sporadically been reported in the Czech Republic, so far only in Enterobacteriaceae and Pseudomonas aeruginosa. In this study, we described the first finding of a KPC-2-producing strain of Klebsiella pneumoniae, which was isolated from a surgical wound swab, decubitus ulcer, and urine of a patient previously hospitalized in Greece. The patient underwent various antibiotic therapies including a colistin treatment. However, after approximately 20 days of the colistin therapy, the strain developed a high-level resistance to this drug. All the isolates were indistinguishable by pulsed field gel electrophoretic analysis and belonged to the international clone ST258, which is typical of KPC-producing K. pneumoniae isolates. The bla (KPC-2) gene was located on a Tn4401a transposon variant. The OmpK35 and OmpK36 genes analysis performed due to the high resistance level of the strains to β-lactams exhibited no changes in their sequence or in their expression when compared with carbapenem-susceptible isolates.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12028125
- 003
- CZ-PrNML
- 005
- 20221006143356.0
- 007
- ta
- 008
- 120817s2011 xr f 000 0eng||
- 009
- AR
- 024 7_
- $a 10.1007/s12223-011-0057-6 $2 doi
- 035 __
- $a (PubMed)21818609
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Hrabák, Jaroslav, $u Department of Microbiology, Faculty of Medicine in Plzeň, Charles University in Prague and University Hospital in Plzeň, Plzeň $d 1981-, $7 xx0025811
- 245 10
- $a KPC-2-producing Klebsiella pneumoniae isolated from a Czech patient previously hospitalized in Greece and in vivo selection of colistin resistance / $c J. Hrabák, J. Niemczyková, E. Chudáčková, M. Fridrichová, V. Studentová, D. Cervená, P. Urbášková, H. Zemličková
- 520 9_
- $a Carbapenemase-producing Gram-negative bacteria peak clinical interest due to their ability to hydrolyze most β-lactams, including carbapenems; moreover, their genes spread through bacterial populations by horizontal transfer. Bacteria with acquired carbapenemase have sporadically been reported in the Czech Republic, so far only in Enterobacteriaceae and Pseudomonas aeruginosa. In this study, we described the first finding of a KPC-2-producing strain of Klebsiella pneumoniae, which was isolated from a surgical wound swab, decubitus ulcer, and urine of a patient previously hospitalized in Greece. The patient underwent various antibiotic therapies including a colistin treatment. However, after approximately 20 days of the colistin therapy, the strain developed a high-level resistance to this drug. All the isolates were indistinguishable by pulsed field gel electrophoretic analysis and belonged to the international clone ST258, which is typical of KPC-producing K. pneumoniae isolates. The bla (KPC-2) gene was located on a Tn4401a transposon variant. The OmpK35 and OmpK36 genes analysis performed due to the high resistance level of the strains to β-lactams exhibited no changes in their sequence or in their expression when compared with carbapenem-susceptible isolates.
- 650 _2
- $a antibakteriální látky $x farmakologie $7 D000900
- 650 _2
- $a kolistin $x farmakologie $7 D003091
- 650 _2
- $a bakteriální léková rezistence $x účinky léků $x genetika $7 D024881
- 650 _2
- $a regulace genové exprese u bakterií $7 D015964
- 650 _2
- $a hospitalizace $7 D006760
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a Klebsiella pneumoniae $x účinky léků $x enzymologie $x genetika $x izolace a purifikace $7 D007711
- 650 _2
- $a mikrobiální testy citlivosti $7 D008826
- 650 _2
- $a poriny $x genetika $7 D018272
- 650 _2
- $a beta-laktamasy $x biosyntéza $x genetika $7 D001618
- 651 _2
- $a Česká republika $7 D018153
- 651 _2
- $a Řecko $7 D006115
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1#
- $a Niemczyková, Jana $u Department of Barteriology and Mykology, Centre of Clinical Laboratories, Institute of Public Health in Ostrava, Havířov $7 _AN077111
- 700 1_
- $a Chudáčková, Eva $u Department of Microbiology, Faculty of Medicine in Plzen, Charles University in Prague and University Hospital in Plzen, Plzeň $7 xx0102187
- 700 1_
- $a Fridrichová, Marta $u National Reference Laboratory for Antibiotics, Natioonal Institute of Public Health, Prague $7 xx0159351
- 700 1#
- $a Študentová, Vendula $u Department of Microbiology, Faculty of Medicine in Plzen, Charles University in Prague and University Hospital in Plzen, Plzeň $7 xx0247066
- 700 1_
- $a Červená, Dana $u Department of Microbiology, Faculty of Medicine in Plzen, Charles University in Prague and University Hospital in Plzen, Plzeň $7 xx0148409
- 700 1_
- $a Urbášková, Pavla $u National Reference Laboratory for Antibiotics, Natioonal Institute of Public Health, Prague $7 nlk20050172889
- 700 1_
- $a Žemličková, Helena, $u National Reference Laboratory for Antibiotics, Natioonal Institute of Public Health, Prague $7 xx0092228 $d 1970-
- 773 0_
- $w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 56, č. 4 (2011), s. 361-365
- 910 __
- $a ABA008 $y m $b online $z 0
- 990 __
- $a 20120817 $b ABA008
- 991 __
- $a 20221006143350 $b ABA008
- 999 __
- $a ok $b bmc $g 950167 $s 785471
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2011 $b 56 $c 4 $d 361-365 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
- GRA __
- $a NS9717 $p MZ0
- LZP __
- $b NLK111 $a Pubmed-20120817/11/04
