Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells

M. Babic, D. Horak, P. Jendelova, V. Herynek, V. Proks, V. Vanecek, P. Lesny, E. Sykova

. 2012 ; 7 () : 1461-1474.

Language English Country New Zealand

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc12034482

Sodium hyaluronate (HA) was associated with dopamine (DPA) and introduced as a coating for maghemite (γ-Fe(2)O(3)) nanoparticles obtained by the coprecipitation of iron(II) and iron(III) chlorides and oxidation with sodium hypochlorite. The effects of the DPA anchorage of HA on the γ-Fe(2)O(3) surface on the physicochemical properties of the resulting colloids were investigated. Nanoparticles coated at three different DPA-HA/γ-Fe(2)O(3) and DPA/HA ratios were chosen for experiments with rat bone marrow mesenchymal stem cells and human chondrocytes. The nanoparticles were internalized into rat bone marrow mesenchymal stem cells via endocytosis as confirmed by Prussian Blue staining. The efficiency of mesenchymal stem cell labeling was analyzed. From among the investigated samples, efficient cell labeling was achieved by using DPA-HA-γ-Fe(2)O(3) nanoparticles with DPA-HA/γ-Fe(2)O(3) = 0.45 (weight/ weight) and DPA/HA = 0.038 (weight/weight) ratios. The particles were used as a contrast agent in magnetic resonance imaging for the labeling and visualization of cells.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc12034482
003      
CZ-PrNML
005      
20160602161323.0
007      
ta
008      
121023s2012 nz f 000 0|eng||
009      
AR
024    7_
$a 10.2147/ijn.s28658 $2 doi
035    __
$a (PubMed)22619506
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a nz
100    1_
$a Babič, Michal $u Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, Prague, Czech Republic; Center for Cell Therapy and Tissue Repair, Charles University, V Uvalu 84, ,Prague $7 xx0109464
245    14
$a The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells / $c M. Babic, D. Horak, P. Jendelova, V. Herynek, V. Proks, V. Vanecek, P. Lesny, E. Sykova
520    9_
$a Sodium hyaluronate (HA) was associated with dopamine (DPA) and introduced as a coating for maghemite (γ-Fe(2)O(3)) nanoparticles obtained by the coprecipitation of iron(II) and iron(III) chlorides and oxidation with sodium hypochlorite. The effects of the DPA anchorage of HA on the γ-Fe(2)O(3) surface on the physicochemical properties of the resulting colloids were investigated. Nanoparticles coated at three different DPA-HA/γ-Fe(2)O(3) and DPA/HA ratios were chosen for experiments with rat bone marrow mesenchymal stem cells and human chondrocytes. The nanoparticles were internalized into rat bone marrow mesenchymal stem cells via endocytosis as confirmed by Prussian Blue staining. The efficiency of mesenchymal stem cell labeling was analyzed. From among the investigated samples, efficient cell labeling was achieved by using DPA-HA-γ-Fe(2)O(3) nanoparticles with DPA-HA/γ-Fe(2)O(3) = 0.45 (weight/ weight) and DPA/HA = 0.038 (weight/weight) ratios. The particles were used as a contrast agent in magnetic resonance imaging for the labeling and visualization of cells.
650    _2
$a zvířata $7 D000818
650    _2
$a buněčná diferenciace $7 D002454
650    _2
$a viabilita buněk $7 D002470
650    _2
$a kultivované buňky $7 D002478
650    _2
$a chondrocyty $x cytologie $7 D019902
650    _2
$a biokompatibilní potahované materiály $x chemie $7 D020099
650    _2
$a kontrastní látky $x diagnostické užití $7 D003287
650    _2
$a dopamin $x chemie $7 D004298
650    _2
$a endocytóza $7 D004705
650    _2
$a ferrokyanidy $7 D005295
650    _2
$a lidé $7 D006801
650    _2
$a kyselina hyaluronová $x chemie $7 D006820
650    _2
$a magnetická rezonanční tomografie $7 D008279
650    _2
$a magnetické nanočástice $x chemie $x diagnostické užití $x ultrastruktura $7 D058185
650    _2
$a mezenchymální kmenové buňky $x cytologie $x účinky léků $7 D059630
650    _2
$a transmisní elektronová mikroskopie $7 D046529
650    _2
$a nanomedicína $7 D050997
650    _2
$a velikost částic $7 D010316
650    _2
$a krysa rodu Rattus $7 D051381
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Horák, Daniel $7 xx0076519 $u Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, Prague, Czech Republic; Center for Cell Therapy and Tissue Repair, Charles University, V Uvalu 84, ,Prague
700    1_
$a Jendelová, Pavla, $d 1965- $7 xx0068971 $u Center for Cell Therapy and Tissue Repair, Charles University, V Uvalu 84; Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, Prague
700    1_
$a Herynek, Vít, $d 1967- $7 xx0069420 $u Center for Cell Therapy and Tissue Repair, Charles University, V Uvalu 84; Institute for Clinical and Experimental Medicine, Videnska 1958, Prague, Czech Republic
700    1_
$a Proks, Vladimír $7 _AN041623 $u Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, Prague, Czech Republic; Center for Cell Therapy and Tissue Repair, Charles University, V Uvalu 84,,Prague
700    1_
$a Vaněček, Václav $7 xx0246192 $u Center for Cell Therapy and Tissue Repair, Charles University, V Uvalu 84; Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, Prague
700    1_
$a Lesný, Petr, $d 1972- $7 xx0060590 $u Center for Cell Therapy and Tissue Repair, Charles University, V Uvalu 84; Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, Prague
700    1_
$a Syková, Eva, $d 1944- $7 jn20000710633 $u Center for Cell Therapy and Tissue Repair, Charles University, V Uvalu 84; Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, Prague
773    0_
$w MED00176143 $t International journal of nanomedicine $x 1178-2013 $g Roč. 7(2012), s. 1461-1474
856    41
$u https://pubmed.ncbi.nlm.nih.gov/22619506 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20121023 $b ABA008
991    __
$a 20160602161058 $b ABA008
999    __
$a ok $b bmc $g 956492 $s 791979
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2012 $b 7 $d 1461-1474 $i 1178-2013 $m International journal of nanomedicine $n Int J Nanomedicine $x MED00176143
LZP    __
$b NLK112 $a Pubmed-20121023

Find record

Citation metrics

Archiving options