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An enzymatic glycosylation of nucleoside analogues using β-galactosidase from Escherichia coli

J. Blažek, P. Jansa, O. Baszczyňski, MM. Kaiser, M. Otmar, M. Krečmerová, M. Drančínský, A. Holý, B. Králová,

. 2012 ; 20 (9) : 3111-8.

Language English Country England, Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc12034615

A new enzymatic method for the synthesis of β-galactosides of nucleosides and acyclic nucleoside analogues has been developed, using β-galactosidase from Escherichia coli as a catalyst and lactose as a sugar donor. The method is very rapid, feasible and last but not least inexpensive. Its applicability has been proven for a broad variety of possible substrates with respect to its scaling up for preparative use. Five new compounds from a series of nucleoside and acyclic nucleoside analogues have been prepared on a scale of several hundred milligrams, in all cases revealing very good results of the method concerning the reproducibility of the reaction yields and simplicity of the purification process.

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