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Evaluation of the use of recombinant Bhlp29.7 in immunoblotting with pig serum as a means to identify herds infected with Brachyspira hyodysenteriae
D. Lobova, J. Prasek, A. Cizek, V. Celer
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
Wiley Online Library (archiv) od 1997-01-01 do 2012-12-31
Odkazy
PubMed
21838749
DOI
10.1111/j.1472-765x.2011.03134.x
Knihovny.cz E-zdroje
- MeSH
- Brachyspira hyodysenteriae imunologie izolace a purifikace MeSH
- dyzenterie diagnóza imunologie veterinární MeSH
- gramnegativní bakteriální infekce diagnóza imunologie veterinární MeSH
- imunoblotting metody MeSH
- lidé MeSH
- lipoproteiny genetika imunologie MeSH
- nemoci prasat diagnóza imunologie mikrobiologie MeSH
- prasata MeSH
- proteiny vnější bakteriální membrány genetika imunologie MeSH
- protilátky bakteriální krev MeSH
- rekombinantní proteiny imunologie MeSH
- séroepidemiologické studie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
AIMS: Aim of the study is to evaluate the use of recombinant Bhlp29.7 in immunoblotting with sera as a means to detect pig herds infected with Brachyspira hyodysenteriae. METHODS AND RESULTS: Sera samples from 789 sows and rectal swabs from 838 pigs of various categories on 22 farms of different size (median 450 animals), production type and history of swine dysentery (SD) were examined. Sera from 378 sows from farms with previous SD history were examined via immunoblotting. Specific antibodies were detected in 79 of these (20.9%). Examination of 411 serum samples from sows and gilts taken on 11 farms without previous history of SD detected specific antibodies in 13 sows and gilts (3.2%). These 13, however, had come from farms where the presence of B. hyodysenteriae was confirmed or SD status was not known. Seroprevalence in herds with previous SD history ranged from 2.5 to 35.7%. B. hyodysenteriae was confirmed on six (27.3%) of 22 monitored farms. CONCLUSIONS: Immunoblotting using recombinant antigen Bhlp29.7 in conjunction with culturing B. hyodysenteriae proved to be a valuable tool for detecting swine herds latently infected with B. hyodysenteriae. SIGNIFICANCE AND IMPACT OF THE STUDY: The use of immunoblotting with recombinant Bhlp29.7 should prove to be a useful adjunct to detecting herds with SD, and hence, it will assist in controlling this important disease.
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- $a AIMS: Aim of the study is to evaluate the use of recombinant Bhlp29.7 in immunoblotting with sera as a means to detect pig herds infected with Brachyspira hyodysenteriae. METHODS AND RESULTS: Sera samples from 789 sows and rectal swabs from 838 pigs of various categories on 22 farms of different size (median 450 animals), production type and history of swine dysentery (SD) were examined. Sera from 378 sows from farms with previous SD history were examined via immunoblotting. Specific antibodies were detected in 79 of these (20.9%). Examination of 411 serum samples from sows and gilts taken on 11 farms without previous history of SD detected specific antibodies in 13 sows and gilts (3.2%). These 13, however, had come from farms where the presence of B. hyodysenteriae was confirmed or SD status was not known. Seroprevalence in herds with previous SD history ranged from 2.5 to 35.7%. B. hyodysenteriae was confirmed on six (27.3%) of 22 monitored farms. CONCLUSIONS: Immunoblotting using recombinant antigen Bhlp29.7 in conjunction with culturing B. hyodysenteriae proved to be a valuable tool for detecting swine herds latently infected with B. hyodysenteriae. SIGNIFICANCE AND IMPACT OF THE STUDY: The use of immunoblotting with recombinant Bhlp29.7 should prove to be a useful adjunct to detecting herds with SD, and hence, it will assist in controlling this important disease.
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