• Je něco špatně v tomto záznamu ?

Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs

L. Planka, R. Srnec, P. Rauser, D. Stary, E. Filova, J. Jancar, J. Juhasova, L. Kren, A. Necas, P. Gal

. 2012 ; 156 (2) : 128-134.

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc12039728

INTRODUCTION: This study describes the results achieved using a combination of allogeneic mesenchymal stem cells (MSCs) with chondrocytes (CHC) and a new scaffold consisting of type-I collagen and chitosan nanofibers in the prevention of partial growth plate arrest after iatrogenic injury in pigs. MATERIAL AND METHODS: The miniature pig was selected as an experimental model to compare the results in the left femoral bones (MSCs and CHC in scaffold transplantation into the iatrogenic partial distal growth plate defect) and right femoral bones (scaffold alone transplantation). The experimental group consisted of 10 animals. Bone marrow from os ilium as the source of MSCs was used. A porous cylinder consisting of 0.5% by weight type-I collagen and 30% by weight chitosan, was the optimal choice. The length of the bone and angular deformity of distal femur after the healing period was measured and the quality and structure of the newly formed cartilage was histologically examined. RESULTS: Transplantation of the composite scaffold in combination with MSCs and chondrocytes led to the prevention of growth disorder and angular deformity in the distal epiphysis of the left femur. Compared to the right (control) femur, tissue similar to hyaline cartilage with signs of columnar organization typical of the growth plate occurred in most cases. CONCLUSIONS: The promising results of this study reveal the new and effective means for the prevention of bone bridge formation after growth plate injury.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc12039728
003      
CZ-PrNML
005      
20140324112528.0
007      
ta
008      
121217s2012 xr o f 000 0|eng||
009      
AR
024    7_
$a 10.5507/bp.2012.041 $2 doi
035    __
$a (PubMed)22837133
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Plánka, Ladislav, $d 1977- $7 xx0081919 $u Clinic of Pediatric Surgery, Orthopedics and Traumatology, Faculty Hospital Brno, Czech Republic. lplanka@seznam.cz
245    10
$a Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs / $c L. Planka, R. Srnec, P. Rauser, D. Stary, E. Filova, J. Jancar, J. Juhasova, L. Kren, A. Necas, P. Gal
520    9
$a INTRODUCTION: This study describes the results achieved using a combination of allogeneic mesenchymal stem cells (MSCs) with chondrocytes (CHC) and a new scaffold consisting of type-I collagen and chitosan nanofibers in the prevention of partial growth plate arrest after iatrogenic injury in pigs. MATERIAL AND METHODS: The miniature pig was selected as an experimental model to compare the results in the left femoral bones (MSCs and CHC in scaffold transplantation into the iatrogenic partial distal growth plate defect) and right femoral bones (scaffold alone transplantation). The experimental group consisted of 10 animals. Bone marrow from os ilium as the source of MSCs was used. A porous cylinder consisting of 0.5% by weight type-I collagen and 30% by weight chitosan, was the optimal choice. The length of the bone and angular deformity of distal femur after the healing period was measured and the quality and structure of the newly formed cartilage was histologically examined. RESULTS: Transplantation of the composite scaffold in combination with MSCs and chondrocytes led to the prevention of growth disorder and angular deformity in the distal epiphysis of the left femur. Compared to the right (control) femur, tissue similar to hyaline cartilage with signs of columnar organization typical of the growth plate occurred in most cases. CONCLUSIONS: The promising results of this study reveal the new and effective means for the prevention of bone bridge formation after growth plate injury.
650    _2
$a zvířata $7 D000818
650    _2
$a kloubní chrupavka $x růst a vývoj $7 D002358
650    _2
$a chitosan $7 D048271
650    _2
$a chondrocyty $x transplantace $7 D019902
650    _2
$a epifýzy $x růst a vývoj $7 D004838
650    _2
$a femur $x chirurgie $x růst a vývoj $7 D005269
650    _2
$a růstová ploténka $x růst a vývoj $7 D006132
650    _2
$a transplantace mezenchymálních kmenových buněk $7 D045164
650    _2
$a nanovlákna $7 D057139
650    _2
$a prasata $7 D013552
650    _2
$a miniaturní prasata $7 D013556
650    _2
$a tkáňové inženýrství $x metody $7 D023822
650    _2
$a tkáňové podpůrné struktury $7 D054457
650    _2
$a fraktury růstové ploténky $7 D000072042
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Srnec, Robert $7 xx0141235 $u Department of Surgery and Orthopaedics, Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno
700    1_
$a Raušer, Petr, $d 1972- $7 mzk2004221783 $u Department of Surgery and Orthopaedics, Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno
700    1_
$a Starý, David $7 xx0102248 $u Clinic of Pediatric Surgery, Orthopedics and Traumatology, Faculty Hospital Brno, Czech Republic
700    1_
$a Filová, Eva $7 xx0171464 $u lnstitute of Experimental Medicine of the Academy of Sciences of the Czech Republic
700    1_
$a Jančář, Josef. $7 xx0013116 $u Institute of Materials Chemistry, Faculty of Chemistry, University of Technology, Brno
700    1_
$a Juhásová, Jana, $d 1979- $7 xx0110087 $u Institute of Animal Physiology and Genetics of the Academy of Sciences of the Czech Republic
700    1_
$a Křen, Leoš, $d 1966- $7 xx0081918 $u Institute of Pathology and Anatomy, Faculty Hospital Brno
700    1_
$a Nečas, Alois, $d 1966- $7 mzk2004229096 $u Department of Surgery and Orthopaedics, Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno
700    1_
$a Gál, Petr, $d 1960- $7 mzk2004237313 $u Clinic of Pediatric Surgery, Orthopedics and Traumatology, Faculty Hospital Brno, Czech Republic
773    0_
$w MED00012606 $t Biomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czech Republic $x 1213-8118 $g Roč. 156, č. 2 (2012), s. 128-134
856    41
$u http://biomed.papers.upol.cz/magno/bio/2012/mn2.php $y domovská stránka časopisu - plný text volně přístupný = fulltext
910    __
$a ABA008 $b A 1502 $c 958 $y 4 $z 0
990    __
$a 20121217 $b ABA008
991    __
$a 20140324112600 $b ABA008
999    __
$a ok $b bmc $g 963280 $s 797316
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2012 $b 156 $c 2 $d 128-134 $i 1213-8118 $m Biomedical papers of the Medical Faculty of the University Palacký, Olomouc Czech Republic $n Biomed. Pap. Fac. Med. Palacký Univ. Olomouc Czech Repub. (Print) $x MED00012606
LZP    __
$b NLK138 $a Pubmed-20121217

Najít záznam

Citační ukazatele

Možnosti archivace