-
Something wrong with this record ?
Tularemia progression accompanied with oxidative stress and antioxidant alteration in spleen and liver of BALB/c mice
M. Pohanka, O. Pavlis, B. Ruttkay-Nedecky, J. Sochor, J. Sobotka, J. Pikula, V. Adam, R. Kizek,
Language English Country Korea (South)
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
ProQuest Central
from 2008-02-01 to 2019-01-31
Open Access Digital Library
from 1995-01-01
Medline Complete (EBSCOhost)
from 2011-02-01 to 1 year ago
Health & Medicine (ProQuest)
from 2008-02-01 to 2019-01-31
- MeSH
- Antioxidants analysis MeSH
- Bacterial Load MeSH
- Francisella tularensis pathogenicity MeSH
- Interferon-gamma blood MeSH
- Interleukin-6 blood MeSH
- Liver microbiology MeSH
- Blood microbiology MeSH
- Malondialdehyde analysis MeSH
- Disease Models, Animal MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Oxidative Stress MeSH
- Spleen microbiology MeSH
- Tularemia microbiology pathology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Francisella tularensis is the causative agent of tularemia. It is an intracellular pathogen with the ability to survive within phagosomes and induce pyroptotic cell death. In this study, we attempted to prove whether oxidative imbalance plays a significant role in tularemia pathogenesis. In our experimental model, we subcutaneously infected female BALB/c mice (dose 10(5) CFU of F. tularensis LVS). Liver, spleen, and blood were collected from mice at regular intervals from days 1-15 after infection. The bacterial burden was assessed by a cultivation test. The burden was unchanging from the 2(nd) to 6(th) day after infection. The bacterial burden corresponded to the plasmatic level of IFN-γ, IL-6, and liver malondialdehyde. After the phase of acute bacteraemia and the innate immunity reaction, the levels of reduced glutathione and total low molecular weight antioxidants decreased significantly and the activity of caspase-3 increased in the liver. The level of reduced glutathione decreased to 25% of the original level, and the total level of low molecular weight antioxidants was less than 50% of the initial amount. The demonstrated effects of tularemia-induced pathology had a more extensive impact on the liver than on the spleen.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc13000687
- 003
- CZ-PrNML
- 005
- 20200430091856.0
- 007
- ta
- 008
- 130108s2012 ko f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s12275-012-1621-8 $2 doi
- 035 __
- $a (PubMed)22752903
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ko
- 100 1_
- $a Pohanka, Miroslav $u Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01, Hradec Kralove, Czech Republic. miroslav.pohanka@gmail.com
- 245 10
- $a Tularemia progression accompanied with oxidative stress and antioxidant alteration in spleen and liver of BALB/c mice / $c M. Pohanka, O. Pavlis, B. Ruttkay-Nedecky, J. Sochor, J. Sobotka, J. Pikula, V. Adam, R. Kizek,
- 520 9_
- $a Francisella tularensis is the causative agent of tularemia. It is an intracellular pathogen with the ability to survive within phagosomes and induce pyroptotic cell death. In this study, we attempted to prove whether oxidative imbalance plays a significant role in tularemia pathogenesis. In our experimental model, we subcutaneously infected female BALB/c mice (dose 10(5) CFU of F. tularensis LVS). Liver, spleen, and blood were collected from mice at regular intervals from days 1-15 after infection. The bacterial burden was assessed by a cultivation test. The burden was unchanging from the 2(nd) to 6(th) day after infection. The bacterial burden corresponded to the plasmatic level of IFN-γ, IL-6, and liver malondialdehyde. After the phase of acute bacteraemia and the innate immunity reaction, the levels of reduced glutathione and total low molecular weight antioxidants decreased significantly and the activity of caspase-3 increased in the liver. The level of reduced glutathione decreased to 25% of the original level, and the total level of low molecular weight antioxidants was less than 50% of the initial amount. The demonstrated effects of tularemia-induced pathology had a more extensive impact on the liver than on the spleen.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a antioxidancia $x analýza $7 D000975
- 650 _2
- $a bakteriální nálož $7 D058491
- 650 _2
- $a krev $x mikrobiologie $7 D001769
- 650 _2
- $a modely nemocí na zvířatech $7 D004195
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a Francisella tularensis $x patogenita $7 D005604
- 650 _2
- $a interferon gama $x krev $7 D007371
- 650 _2
- $a interleukin-6 $x krev $7 D015850
- 650 _2
- $a játra $x mikrobiologie $7 D008099
- 650 _2
- $a malondialdehyd $x analýza $7 D008315
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a myši inbrední BALB C $7 D008807
- 650 _2
- $a oxidační stres $7 D018384
- 650 _2
- $a slezina $x mikrobiologie $7 D013154
- 650 _2
- $a tularemie $x mikrobiologie $x patologie $7 D014406
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Pavliš, Oto $7 xx0135660
- 700 1_
- $a Ruttkay-Nedecky, Branislav
- 700 1_
- $a Sochor, Jiri
- 700 1_
- $a Sobotka, Jakub
- 700 1_
- $a Pikula, Jiri
- 700 1_
- $a Adam, Vojtěch $7 xx0064599
- 700 1_
- $a Kizek, Rene
- 773 0_
- $w MED00181724 $t Journal of microbiology (Seoul, Korea) $x 1976-3794 $g Roč. 50, č. 3 (2012), s. 401-8
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/22752903 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20130108 $b ABA008
- 991 __
- $a 20200430091851 $b ABA008
- 999 __
- $a ok $b bmc $g 963469 $s 798851
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2012 $b 50 $c 3 $d 401-8 $i 1976-3794 $m The journal of microbiology $n J Microbiol $x MED00181724
- LZP __
- $a Pubmed-20130108