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The cathepsin-D expression in the cyto-invasive stages of coccidia in intestinal mucosa of chickens, and the relevance of this proteinase for intracellular penetration and spreading of coccidia in host tissues
K. Rostislav, B. Petr, C. Frantisek, K. Oldrich
Jazyk angličtina Země Česko
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2001
Free Medical Journals
od 1998
ROAD: Directory of Open Access Scholarly Resources
od 2001
PubMed
12415638
DOI
10.5507/bp.2001.004
Knihovny.cz E-zdroje
- MeSH
- imunohistochemie MeSH
- kathepsin D metabolismus MeSH
- kokcidióza enzymologie parazitologie veterinární MeSH
- kur domácí * MeSH
- nemoci drůbeže enzymologie parazitologie MeSH
- střevní sliznice enzymologie parazitologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The Cathepsin-D proteinase was demonstrated immunohistochemically in cases of intestinal coccidiosis in chickens; its expression concerned almost the cyto-invasive stages of reproductive cycle of several strains of coccidia (merozoites of the II-nd generation and also microgametes). The authors consider Cathepsin-D as a potentional "penetration/spreading factor", being significant for intracellular invasion and spreading of coccidia into host cells. In diagnostic practice, an immunohistochemical demonstration of Cathepsin-D is a relatively simple and sensitive method, which brings information on the extent of parasitic lesions, and on participation of particular evolutive forms of coccidial life cycle; it may also be useful in proving isolated coccidia in the intestinal epithelium, poorly recognized in routine HE preparation. Some Cathepsin-D protease reactivity in intestinal Cryptosporidiosis is also briefly mentioned. The examined cases of cerebral toxoplasmosis and primary acanthamoebic meningoencephalitis (PAME) showed in above mentioned methods negative results.
Biofarm Research Institute for Biopharmacy and Veterinary therapeutics Prague
Institute of Pathology Medical Faculty Palacký University Olomouc
Citace poskytuje Crossref.org
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