Retention characteristics of selected synthetic 5'-terminal phosphate absent penta-nucleotides containing adenine, guanine, and thymine were studied in relation to their sequence by hydrophilic interaction chromatography and ion-interaction reversed-phase liquid chromatography. The organic solvent content, pH, and buffer concentration in mobile phases were evaluated as influential separation conditions. Data demonstrate that both compared chromatographic modes can be used to separate synthetic penta-nucleotides according to their nucleotide composition. Moreover, reversed-phase liquid chromatography allows separation according to their sequence. We have found a simple linear additive model to describe the retention order in both separation modes in regard to their sequence. In hydrophilic interaction chromatography, the retention behavior is controlled primarily by the hydrophilicity of involved nucleotides and minimally by their sequence position. For reversed-phase liquid chromatography, the nucleotide hydrophobicity plays an important role in their retention properties and the influence of their location in sequence on the retention increases toward the center and decreases toward the termini. Our results show that the penta-nucleotide sequence, and thus its spatial arrangement induced by the surrounding environment, is highly related to the retention properties, so it may be hypothetically used to read the sequence from the retention properties acquired under particular separation conditions.

Faculty of Science Department of Chemistry Masaryk University Brno Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc13024131

003      

CZ-PrNML

005      

20130710121344.0

007      

ta

008      

130703s2012 gw f 000 0|eng||

009      

AR

024    7_

$a 10.1002/jssc.201200482 $2 doi

035    __

$a (PubMed)23175142

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a gw

100    1_

$a Bittová, Miroslava $u Faculty of Science, Department of Chemistry, Masaryk University, Brno, Czech Republic.

245    10

$a Toward reading the sequence of short oligonucleotides from their retention factors obtained by means of hydrophilic interaction chromatography and ion-interaction reversed-phase liquid chromatography / $c M. Bittová, J. Havliš, H. Fuksová, B. Vrbková, L. Trnková,

520    9_

$a Retention characteristics of selected synthetic 5'-terminal phosphate absent penta-nucleotides containing adenine, guanine, and thymine were studied in relation to their sequence by hydrophilic interaction chromatography and ion-interaction reversed-phase liquid chromatography. The organic solvent content, pH, and buffer concentration in mobile phases were evaluated as influential separation conditions. Data demonstrate that both compared chromatographic modes can be used to separate synthetic penta-nucleotides according to their nucleotide composition. Moreover, reversed-phase liquid chromatography allows separation according to their sequence. We have found a simple linear additive model to describe the retention order in both separation modes in regard to their sequence. In hydrophilic interaction chromatography, the retention behavior is controlled primarily by the hydrophilicity of involved nucleotides and minimally by their sequence position. For reversed-phase liquid chromatography, the nucleotide hydrophobicity plays an important role in their retention properties and the influence of their location in sequence on the retention increases toward the center and decreases toward the termini. Our results show that the penta-nucleotide sequence, and thus its spatial arrangement induced by the surrounding environment, is highly related to the retention properties, so it may be hypothetically used to read the sequence from the retention properties acquired under particular separation conditions.

650    _2

$a vysokoúčinná kapalinová chromatografie $x metody $7 D002851

650    _2

$a chromatografie kapalinová $x metody $7 D002853

650    _2

$a chromatografie s reverzní fází $x metody $7 D056148

650    _2

$a koncentrace vodíkových iontů $7 D006863

650    _2

$a hydrofobní a hydrofilní interakce $7 D057927

650    _2

$a kinetika $7 D007700

650    _2

$a oligonukleotidy $x chemická syntéza $x chemie $7 D009841

655    _2

$a hodnotící studie $7 D023362

655    _2

$a časopisecké články $7 D016428

655    _2

$a práce podpořená grantem $7 D013485

700    1_

$a Havliš, Jan $u -

700    1_

$a Fuksová, Hana $u -

700    1_

$a Vrbková, Blanka $u -

700    1_

$a Trnková, Libuše $u -

773    0_

$w MED00006463 $t Journal of separation science $x 1615-9314 $g Roč. 35, č. 22 (2012), s. 3227-34

856    41

$u https://pubmed.ncbi.nlm.nih.gov/23175142 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20130703 $b ABA008

991    __

$a 20130710121807 $b ABA008

999    __

$a ok $b bmc $g 987811 $s 822511

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2012 $b 35 $c 22 $d 3227-34 $i 1615-9314 $m Journal of separation science $n J Sep Sci $x MED00006463

LZP    __

$a Pubmed-20130703