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Quantification of abscisic Acid, cytokinin, and auxin content in salt-stressed plant tissues
PI. Dobrev, R. Vankova,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- cytokininy analýza izolace a purifikace MeSH
- fyziologický stres * MeSH
- hmotnostní spektrometrie MeSH
- kořeny rostlin chemie metabolismus MeSH
- kyselina abscisová analýza izolace a purifikace MeSH
- kyseliny indoloctové analýza izolace a purifikace MeSH
- listy rostlin chemie metabolismus MeSH
- rostlinné extrakty chemie MeSH
- rostliny chemie metabolismus MeSH
- salinita * MeSH
- tolerance k soli fyziologie MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Plant hormones cytokinins, auxin (indole-3-acetic acid), and abscisic acid are central to regulation of plant growth and defence to abiotic stresses such as salinity. Quantification of the hormone levels and determination of their ratios can reveal different plant strategies to cope with the stress, e.g., suppression of growth or mobilization of plant metabolism. This chapter describes a procedure enabling such quantification. Due to the high variability of these hormones in plant tissues, it is advantageous to determine their content in the same sample. Reverse phase and ion exchange chromatography allows separation of the individual hormone fractions. Hormones as well as their metabolites can be identified and quantified by LC/MS.
Citace poskytuje Crossref.org
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