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Bilateral changes of cannabinoid receptor type 2 protein and mRNA in the dorsal root ganglia of a rat neuropathic pain model
IH. Svízenská, V. Brázda, I. Klusáková, P. Dubový,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1953 to 1 year ago
SAGE Publications Journals
from 1999-01-01 to 2015-12-31
SAGE Journals
from 2013-07-01 to 2013-07-31
- MeSH
- Behavior, Animal MeSH
- Rats MeSH
- RNA, Messenger genetics metabolism MeSH
- Disease Models, Animal MeSH
- Sciatic Nerve injuries MeSH
- Neuralgia genetics metabolism pathology MeSH
- Rats, Wistar MeSH
- Receptor, Cannabinoid, CB2 genetics metabolism MeSH
- Gene Expression Regulation * MeSH
- Ganglia, Spinal metabolism pathology MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Cannabinoid receptor type 2 (CB2R) plays a critical role in nociception. In contrast to cannabinoid receptor type 1 ligands, CB2R agonists do not produce undesirable central nervous system effects and thus promise to treat neuropathic pain that is often resistant to medical therapy. In the study presented here, we evaluated the bilateral distribution of the CB2R protein and messenger RNA (mRNA) in rat dorsal root ganglia (DRG) after unilateral peripheral nerve injury using immunohistochemistry, western blot, and in situ hybridization analysis. Unilateral chronic constriction injury (CCI) of the sciatic nerve induced neuropathic pain behavior and bilateral elevation of both CB2R protein and mRNA in lumbar L4-L5 as well as cervical C7-C8 DRG when compared with naive animals. CB2R protein and mRNA were increased not only in DRG neurons but also in satellite glial cells. The fact that changes appear bilaterally and (albeit at a lower level) even in the remote cervical DRG can be related to propagation of neuroinflammation alongside the neuraxis and to the neuroprotective effects of CB2R.
References provided by Crossref.org
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