-
Je něco špatně v tomto záznamu ?
LC-ESI-MS/MS method for oxidative stress multimarker screening in the exhaled breath condensate of asbestosis/silicosis patients
K. Syslová, P. Kačer, M. Kuzma, A. Pankrácová, Z. Fenclová, S. Vlčková, J. Lebedová, D. Pelclová,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- azbestóza metabolismus MeSH
- biologické markery analýza MeSH
- chromatografie kapalinová MeSH
- dechové testy MeSH
- dospělí MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací metody MeSH
- lidé MeSH
- lyofilizace MeSH
- mladý dospělý MeSH
- oxidační stres * MeSH
- silikóza metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The sensitive assay method was developed for a parallel, rapid and precise determination of the most prominent oxidative stress biomarkers: 8-iso-prostaglandin F(2α) a lipid oxidation biomarker, o-tyrosine an amino acid oxidation biomarker and 8-hydroxy-2'-deoxy-guanosine a nucleic acid oxidation biomarker. The method consisted of a pre-treatment part, freeze drying (lyophilization), serving the purpose of biomarkers concentration from the exhaled breath condensate and detection method LC-ESI-MS/MS, where the selected reaction-monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification. The developed method is characterized by the following parameters: the precision was higher than 84.3% and the mean accuracy (relative error) was determined lower than 11.6%. The method was tested on samples obtained from patients diagnosed with asbestosis and silicosis, occupational diseases induced by oxidative stress, and then compared with samples from healthy subjects. The difference in biomarkers' concentration levels found between the two groups was statistically significant.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc13031959
- 003
- CZ-PrNML
- 005
- 20250204152601.0
- 007
- ta
- 008
- 131002s2010 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1088/1752-7155/4/1/017104 $2 doi
- 035 __
- $a (PubMed)21386209
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Syslová, K $u Institute of Chemical Technology, Technická 5, 166 28 Prague 6, Czech Republic. Institute of Microbiology, Vídeňská 1083, 142 20 Prague 4, Czech Republic.
- 245 10
- $a LC-ESI-MS/MS method for oxidative stress multimarker screening in the exhaled breath condensate of asbestosis/silicosis patients / $c K. Syslová, P. Kačer, M. Kuzma, A. Pankrácová, Z. Fenclová, S. Vlčková, J. Lebedová, D. Pelclová,
- 520 9_
- $a The sensitive assay method was developed for a parallel, rapid and precise determination of the most prominent oxidative stress biomarkers: 8-iso-prostaglandin F(2α) a lipid oxidation biomarker, o-tyrosine an amino acid oxidation biomarker and 8-hydroxy-2'-deoxy-guanosine a nucleic acid oxidation biomarker. The method consisted of a pre-treatment part, freeze drying (lyophilization), serving the purpose of biomarkers concentration from the exhaled breath condensate and detection method LC-ESI-MS/MS, where the selected reaction-monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification. The developed method is characterized by the following parameters: the precision was higher than 84.3% and the mean accuracy (relative error) was determined lower than 11.6%. The method was tested on samples obtained from patients diagnosed with asbestosis and silicosis, occupational diseases induced by oxidative stress, and then compared with samples from healthy subjects. The difference in biomarkers' concentration levels found between the two groups was statistically significant.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a azbestóza $x metabolismus $7 D001195
- 650 _2
- $a biologické markery $x analýza $7 D015415
- 650 _2
- $a dechové testy $7 D001944
- 650 _2
- $a chromatografie kapalinová $7 D002853
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a lyofilizace $7 D005612
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 12
- $a oxidační stres $7 D018384
- 650 _2
- $a silikóza $x metabolismus $7 D012829
- 650 _2
- $a hmotnostní spektrometrie s elektrosprejovou ionizací $x metody $7 D021241
- 650 _2
- $a mladý dospělý $7 D055815
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Kačer, P
- 700 1_
- $a Kuzma, M
- 700 1_
- $a Pankrácová, A
- 700 1_
- $a Fenclová, Zdeňka, $d 1958- $7 xx0074215
- 700 1_
- $a Vlčková, S
- 700 1_
- $a Lebedová, J
- 700 1_
- $a Pelclová, D
- 773 0_
- $w MED00182181 $t Journal of breath research $x 1752-7163 $g Roč. 4, č. 1 (2010), s. 017104
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/21386209 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20131002 $b ABA008
- 991 __
- $a 20250204152600 $b ABA008
- 999 __
- $a ok $b bmc $g 996046 $s 830404
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2010 $b 4 $c 1 $d 017104 $i 1752-7163 $m Journal of breath research $n J Breath Res $x MED00182181
- LZP __
- $a Pubmed-20131002
