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A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA
I Hilgert, P Stolba, H Kristofova, I Stefanova, B Bendlova, M Lebl, V Horejsi
Language English Country United States
Grant support
IZ8
MZ0
CEP Register
- MeSH
- C-Peptide analysis immunology MeSH
- Hybridomas immunology MeSH
- Humans MeSH
- Molecular Sequence Data MeSH
- Antibodies, Monoclonal * MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Peptide Fragments chemistry immunology MeSH
- Radioimmunoassay MeSH
- Amino Acid Sequence MeSH
- Antibody Specificity MeSH
- Cross Reactions MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
BALB/c, (BALB/c x B10.A)F1 and (BALB/c x B10)F1 hybrid mice were immunized with C-peptide of human proinsulin. The (BALB/c x B10.A)F1 hybrids were the best responders and yielded 3 hybridomas secreting specific monoclonal antibodies. One of them, C-PEP-01, bound the C-peptide with high affinity (Kas = 1.1 x 10(9) l/mol), cross-reacted fully with human proinsulin but not with insulin, glucagon or somatostatin and apparently recognized the regions of C-peptide comprising amino acid residues 8-13 and 25-31. A RIA system could be set up employing this monoclonal antibody suitable for estimation of C-peptide concentrations in a diagnostically useful range (1-50 ng/ml).
Institute of Molecular Genetics Czechoslovak Academy of Sciences Prague
Institute of Organic Chemistry and biochemistry Czechoslovak Academy of Sciences Prague
References provided by Crossref.org
Literatura
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