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Adhesion, growth, and maturation of vascular smooth muscle cells on low-density polyethylene grafted with bioactive substances
M. Parizek, N. Slepickova Kasalkova, L. Bacakova, Z. Svindrych, P. Slepicka, M. Bacakova, V. Lisa, V. Svorcik,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 2013
PubMed Central
from 2013
Europe PubMed Central
from 2013
ProQuest Central
from 2013
Open Access Digital Library
from 2001-01-01
Open Access Digital Library
from 2012-12-04
Open Access Digital Library
from 2013-01-01
CINAHL Plus with Full Text (EBSCOhost)
from 2013-01-01
Medline Complete (EBSCOhost)
from 2013-01-01
Health & Medicine (ProQuest)
from 2013
Wiley-Blackwell Open Access Titles
from 2001
ROAD: Directory of Open Access Scholarly Resources
from 2013
PubMed
23586032
DOI
10.1155/2013/371430
Knihovny.cz E-resources
- MeSH
- Aorta cytology drug effects MeSH
- Biocompatible Materials chemistry pharmacology MeSH
- Cell Adhesion drug effects MeSH
- Glycine chemistry pharmacology MeSH
- Rats MeSH
- Cells, Cultured MeSH
- Myocytes, Smooth Muscle cytology drug effects MeSH
- Polyethylene chemistry pharmacology MeSH
- Polyethylene Glycols chemistry pharmacology MeSH
- Surface Properties MeSH
- Cell Proliferation drug effects MeSH
- Serum Albumin, Bovine chemistry pharmacology MeSH
- Muscle, Smooth, Vascular cytology drug effects MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The attractiveness of synthetic polymers for cell colonization can be affected by physical, chemical, and biological modification of the polymer surface. In this study, low-density polyethylene (LDPE) was treated by an Ar(+) plasma discharge and then grafted with biologically active substances, namely, glycine (Gly), polyethylene glycol (PEG), bovine serum albumin (BSA), colloidal carbon particles (C), or BSA+C. All modifications increased the oxygen content, the wettability, and the surface free energy of the materials compared to the pristine LDPE, but these changes were most pronounced in LDPE with Gly or PEG, where all the three values were higher than in the only plasma-treated samples. When seeded with vascular smooth muscle cells (VSMCs), the Gly- or PEG-grafted samples increased mainly the spreading and concentration of focal adhesion proteins talin and vinculin in these cells. LDPE grafted with BSA or BSA+C showed a similar oxygen content and similar wettability, as the samples only treated with plasma, but the nano- and submicron-scale irregularities on their surface were more pronounced and of a different shape. These samples promoted predominantly the growth, the formation of a confluent layer, and phenotypic maturation of VSMC, demonstrated by higher concentrations of contractile proteins alpha-actin and SM1 and SM2 myosins. Thus, the behavior of VSMC on LDPE can be regulated by the type of bioactive substances that are grafted.
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