Methanolic extract from lyophilized roots of Impatiens glandulifera Royle was analyzed by high performance liquid chromatography using DAD and FLD detection and this revealed one dominant highly fluorescent very unstable substance. The stability of this derivative is strongly dependent on the plant material drying procedure and extraction procedure used. The structure of the substance was established as 1,2,4-trihydroxynaphthalene-1-O-glucoside (THNG) according LC-MS and NMR measurements. When lyophilized plant material was extracted with methanol an almost four times higher amount of THNG was found in the extract, compared to the amount of 2-hydroxy-1,4-naphthoquinone obtained, while in the case of the same lyophilized plant material extracted with water there was no THNG in the extract. The main compounds in this case was 2-hydroxy-1,4-naphthoquinone. In the plant material dried at the laboratory temperature and extracted by methanol there are only traces of THNG.

000      

00000naa a2200000 a 4500

001      

bmc14050904

003      

CZ-PrNML

005      

20140411105747.0

007      

ta

008      

140401s2013 sz f 000 0|eng||

009      

AR

024    7_

$a 10.3390/molecules18078429 $2 doi

035    __

$a (PubMed)23867652

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a sz

100    1_

$a Tříska, Jan

245    10

$a Separation and identification of 1,2,4-trihydroxynaphthalene-1-O-glucoside in Impatiens glandulifera Royle / $c J. Tříska, N. Vrchotová, J. Sýkora, M. Moos,

520    9_

$a Methanolic extract from lyophilized roots of Impatiens glandulifera Royle was analyzed by high performance liquid chromatography using DAD and FLD detection and this revealed one dominant highly fluorescent very unstable substance. The stability of this derivative is strongly dependent on the plant material drying procedure and extraction procedure used. The structure of the substance was established as 1,2,4-trihydroxynaphthalene-1-O-glucoside (THNG) according LC-MS and NMR measurements. When lyophilized plant material was extracted with methanol an almost four times higher amount of THNG was found in the extract, compared to the amount of 2-hydroxy-1,4-naphthoquinone obtained, while in the case of the same lyophilized plant material extracted with water there was no THNG in the extract. The main compounds in this case was 2-hydroxy-1,4-naphthoquinone. In the plant material dried at the laboratory temperature and extracted by methanol there are only traces of THNG.

650    _2

$a vysokoúčinná kapalinová chromatografie $7 D002851

650    _2

$a glukosidy $x chemie $x izolace a purifikace $7 D005960

650    _2

$a Impatiens $x chemie $7 D029069

650    _2

$a magnetická rezonanční spektroskopie $x metody $7 D009682

650    _2

$a hmotnostní spektrometrie $7 D013058

650    _2

$a naftoly $x chemie $x izolace a purifikace $7 D009284

650    _2

$a rostlinné extrakty $x chemie $7 D010936

650    _2

$a kořeny rostlin $x chemie $7 D018517

655    _2

$a časopisecké články $7 D016428

655    _2

$a práce podpořená grantem $7 D013485

700    1_

$a Vrchotová, Naděžda $u -

700    1_

$a Sýkora, Jan $u -

700    1_

$a Moos, Martin $u -

773    0_

$w MED00180394 $t Molecules (Basel, Switzerland) $x 1420-3049 $g Roč. 18, č. 7 (2013), s. 8429-39

856    41

$u https://pubmed.ncbi.nlm.nih.gov/23867652 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20140401 $b ABA008

991    __

$a 20140411105837 $b ABA008

999    __

$a ok $b bmc $g 1018040 $s 849484

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2013 $b 18 $c 7 $d 8429-39 $i 1420-3049 $m Molecules $n Molecules $x MED00180394

LZP    __

$a Pubmed-20140401