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Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I transcription
S. Yildirim, E. Castano, M. Sobol, VV. Philimonenko, R. Dzijak, T. Venit, P. Hozák,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1966 to 6 months ago
Open Access Digital Library
from 1853-01-01
Open Access Digital Library
from 1853-01-01
PubMed
23591814
DOI
10.1242/jcs.123661
Knihovny.cz E-resources
- MeSH
- Cell Nucleolus genetics metabolism MeSH
- Chromosomal Proteins, Non-Histone genetics metabolism MeSH
- DNA-Binding Proteins genetics metabolism MeSH
- Phosphatidylinositol 4,5-Diphosphate genetics metabolism MeSH
- Transcription, Genetic genetics MeSH
- HeLa Cells MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- RNA Precursors genetics metabolism MeSH
- Promoter Regions, Genetic genetics MeSH
- RNA Polymerase I genetics metabolism MeSH
- Pol1 Transcription Initiation Complex Proteins genetics metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
RNA polymerase I (Pol I) transcription is essential for the cell cycle, growth and protein synthesis in eukaryotes. In the present study, we found that phosphatidylinositol 4,5-bisphosphate (PIP2) is a part of the protein complex on the active ribosomal promoter during transcription. PIP2 makes a complex with Pol I and the Pol I transcription factor UBF in the nucleolus. PIP2 depletion reduces Pol I transcription, which can be rescued by the addition of exogenous PIP2. In addition, PIP2 also binds directly to the pre-rRNA processing factor fibrillarin (Fib), and co-localizes with nascent transcripts in the nucleolus. PIP2 binding to UBF and Fib modulates their binding to DNA and RNA, respectively. In conclusion, PIP2 interacts with a subset of Pol I transcription machinery, and promotes Pol I transcription.
References provided by Crossref.org
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