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Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I transcription
S. Yildirim, E. Castano, M. Sobol, VV. Philimonenko, R. Dzijak, T. Venit, P. Hozák,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Free Medical Journals
od 1966 do Před 6 měsíci
Open Access Digital Library
od 1853-01-01
Open Access Digital Library
od 1853-01-01
PubMed
23591814
DOI
10.1242/jcs.123661
Knihovny.cz E-zdroje
- MeSH
- buněčné jadérko genetika metabolismus MeSH
- chromozomální proteiny, nehistonové genetika metabolismus MeSH
- DNA vazebné proteiny genetika metabolismus MeSH
- fosfatidylinositol-4,5-difosfát genetika metabolismus MeSH
- genetická transkripce genetika MeSH
- HeLa buňky MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- prekurzory RNA genetika metabolismus MeSH
- promotorové oblasti (genetika) genetika MeSH
- RNA-polymerasa I genetika metabolismus MeSH
- transkripční iniciační komplex Pol1 - proteiny genetika metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
RNA polymerase I (Pol I) transcription is essential for the cell cycle, growth and protein synthesis in eukaryotes. In the present study, we found that phosphatidylinositol 4,5-bisphosphate (PIP2) is a part of the protein complex on the active ribosomal promoter during transcription. PIP2 makes a complex with Pol I and the Pol I transcription factor UBF in the nucleolus. PIP2 depletion reduces Pol I transcription, which can be rescued by the addition of exogenous PIP2. In addition, PIP2 also binds directly to the pre-rRNA processing factor fibrillarin (Fib), and co-localizes with nascent transcripts in the nucleolus. PIP2 binding to UBF and Fib modulates their binding to DNA and RNA, respectively. In conclusion, PIP2 interacts with a subset of Pol I transcription machinery, and promotes Pol I transcription.
Citace poskytuje Crossref.org
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