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A small portion of plastid transcripts is polyadenylated in the flagellate Euglena gracilis

K. Záhonová, L. Hadariová, R. Vacula, V. Yurchenko, M. Eliáš, J. Krajčovič, M. Vesteg,

. 2014 ; 588 (5) : 783-8.

Language English Country Netherlands

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc14063739

Euglena gracilis possesses secondary plastids of green algal origin. In this study, E. gracilis expressed sequence tags (ESTs) derived from polyA-selected mRNA were searched and several ESTs corresponding to plastid genes were found. PCR experiments failed to detect SL sequence at the 5'-end of any of these transcripts, suggesting plastid origin of these polyadenylated molecules. Quantitative PCR experiments confirmed that polyadenylation of transcripts occurs in the Euglena plastids. Such transcripts have been previously observed in primary plastids of plants and algae as low-abundance intermediates of transcript degradation. Our results suggest that a similar mechanism exists in secondary plastids.

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$a Euglena gracilis possesses secondary plastids of green algal origin. In this study, E. gracilis expressed sequence tags (ESTs) derived from polyA-selected mRNA were searched and several ESTs corresponding to plastid genes were found. PCR experiments failed to detect SL sequence at the 5'-end of any of these transcripts, suggesting plastid origin of these polyadenylated molecules. Quantitative PCR experiments confirmed that polyadenylation of transcripts occurs in the Euglena plastids. Such transcripts have been previously observed in primary plastids of plants and algae as low-abundance intermediates of transcript degradation. Our results suggest that a similar mechanism exists in secondary plastids.
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$a Hadariová, Lucia $u Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
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$a Vacula, Rostislav $u Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
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$a Yurchenko, Vyacheslav $u Department of Biology and Ecology, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.
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$a Eliáš, Marek $u Department of Biology and Ecology, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.
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$a Krajčovič, Juraj $u Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
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$a Vesteg, Matej $u Department of Biology and Ecology, Faculty of Science, University of Ostrava, Ostrava, Czech Republic; Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia. Electronic address: vesteg@fns.uniba.sk.
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