-
Something wrong with this record ?
InPouch™ TF-Feline medium is not specific for Tritrichomonas foetus
V. Ceplecha, M. Svoboda, I. Cepička, R. Husník, K. Horáčková, V. Svobodová,
Language English Country Netherlands
Document type Journal Article
- MeSH
- Feces parasitology MeSH
- Cats MeSH
- Culture Media MeSH
- Cat Diseases diagnosis parasitology MeSH
- Protozoan Infections, Animal diagnosis parasitology MeSH
- Tritrichomonas foetus isolation & purification MeSH
- Animals MeSH
- Check Tag
- Cats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Trichomonads are a group of anaerobic flagellates. Two species of intestinal trichomonads have been described in cats - pathogenic Tritrichomonas foetus and probably harmless Pentatrichomonas hominis. InPouch™ TF-Feline medium (Bio-Med Diagnostics, White City, Oregon, USA) is considered the gold standard for diagnosis of T. foetus infection in cats. It is commercially available, relatively cheap and easy-to-use. The medium is intended to be highly specific to T. foetus as morphologically similar Pentatrichomonas hominis and Giardia sp. do not survive here longer than 24h. In this study we successfully cultivated P. hominis in InPouch™ TF-Feline medium for 3 days after inoculation with cat faeces. The identity of the organism was assessed by sequencing of SSU rDNA and ITS region. Possible coinfection with T. foetus was ruled out using Tritrichomonas-specific PCR. Our results suggest possible misdiagnosis of tritrichomonosis in cats using InPouch™ TF-Feline medium. PCR-based verification of culture-positive samples prior the potentially neurotoxic ronidazole treatment is recommended.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc14064183
- 003
- CZ-PrNML
- 005
- 20140707113215.0
- 007
- ta
- 008
- 140704s2013 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.vetpar.2013.04.015 $2 doi
- 035 __
- $a (PubMed)23643452
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Ceplecha, Václav $u Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech Republic. v.ceplecha@gmail.com
- 245 10
- $a InPouch™ TF-Feline medium is not specific for Tritrichomonas foetus / $c V. Ceplecha, M. Svoboda, I. Cepička, R. Husník, K. Horáčková, V. Svobodová,
- 520 9_
- $a Trichomonads are a group of anaerobic flagellates. Two species of intestinal trichomonads have been described in cats - pathogenic Tritrichomonas foetus and probably harmless Pentatrichomonas hominis. InPouch™ TF-Feline medium (Bio-Med Diagnostics, White City, Oregon, USA) is considered the gold standard for diagnosis of T. foetus infection in cats. It is commercially available, relatively cheap and easy-to-use. The medium is intended to be highly specific to T. foetus as morphologically similar Pentatrichomonas hominis and Giardia sp. do not survive here longer than 24h. In this study we successfully cultivated P. hominis in InPouch™ TF-Feline medium for 3 days after inoculation with cat faeces. The identity of the organism was assessed by sequencing of SSU rDNA and ITS region. Possible coinfection with T. foetus was ruled out using Tritrichomonas-specific PCR. Our results suggest possible misdiagnosis of tritrichomonosis in cats using InPouch™ TF-Feline medium. PCR-based verification of culture-positive samples prior the potentially neurotoxic ronidazole treatment is recommended.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a nemoci koček $x diagnóza $x parazitologie $7 D002371
- 650 _2
- $a kočky $7 D002415
- 650 _2
- $a kultivační média $7 D003470
- 650 _2
- $a feces $x parazitologie $7 D005243
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a protozoální infekce zvířat $x diagnóza $x parazitologie $7 D011529
- 650 _2
- $a Tritrichomonas foetus $x izolace a purifikace $7 D016835
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Svoboda, Miroslav
- 700 1_
- $a Cepička, Ivan
- 700 1_
- $a Husník, Roman
- 700 1_
- $a Horáčková, Kateřina
- 700 1_
- $a Svobodová, Vlasta
- 773 0_
- $w MED00006161 $t Veterinary parasitology $x 1873-2550 $g Roč. 196, č. 3-4 (2013), s. 503-5
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/23643452 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20140704 $b ABA008
- 991 __
- $a 20140707113503 $b ABA008
- 999 __
- $a ok $b bmc $g 1031667 $s 862915
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2013 $b 196 $c 3-4 $d 503-5 $i 1873-2550 $m Veterinary parasitology $n Vet Parasitol $x MED00006161
- LZP __
- $a Pubmed-20140704
