Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Immunosuppressive protocol with delayed use of low-dose tacrolimus after aortic transplantation suppresses donor-specific anti-MHC class I and class II antibody production in rats

I. Matia, P. Fellmer, K. Splith, M. Varga, M. Adamec, I. Kämmerer, L. Feldbrügge, F. Krenzien, HM. Hau, G. Atanasov, M. Schmelzle, S. Jonas,

. 2014 ; 19 (-) : 225-32.

Language English Country Poland

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc14074213

BACKGROUND: Arterial allografts are used as vascular conduits in the treatment of prosthetic graft infection. Immunosuppression decreases their rupture risk rate. However, immunosuppression can be unprofitable in florid infection. Previously, we confirmed inhibition of cell-mediated destruction of rat aortic grafts by delayed use of tacrolimus. In this work, we studied the influence of this protocol on the antibody-mediated rejection. MATERIAL AND METHODS: Flow cytometry was used for the retrospective analysis of day 0, 14, and 30 sera obtained from Lewis rat recipients of isogeneic fresh infrarenal aortic grafts (group A) or Brown-Norway rat aortic grafts (group B,C,D) for the presence of donor-specific anti-MHC class I and II antibodies. Tacrolimus in daily dose of 0.2 mg/kg was administered from day 1 to day 30 (group C) or from day 7 to day 30 (group D). RESULTS: Inhibition of fluorescence-labeled anti-BN MHC class I and MHC class II antibodies binding to BN-splenocytes was observed only by day 14 and day 30 sera of allogeneic non-immunosuppressed Lewis rats (group B). The day 30 sera significantly decreased anti-MHC I (42±3%) and anti-MHC II antibody binding (56±3%) compared to day 0 (76±9%, p=0.005 and 79±5%, p=0.003, respectively). Deposition of immunoglobulins G into the tunica media was observed only in non-immunosuppressed aortic allografts on day 30. CONCLUSIONS: Fresh aortic allografts induce donor-specific anti-MHC class I and anti-MHC class II antibody production. Delayed administration of tacrolimus completely suppressed antibody production and antibody-mediated destruction of aortic allografts.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc14074213
003      
CZ-PrNML
005      
20141007102303.0
007      
ta
008      
141006s2014 pl f 000 0|eng||
009      
AR
024    7_
$a 10.12659/AOT.889870 $2 doi
035    __
$a (PubMed)24815872
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a pl
100    1_
$a Matia, Ivan $u Department of Visceral, Transplantation, Thoracic and Vascular Surgery, University of Leipzig, Leipzig, Germany.
245    10
$a Immunosuppressive protocol with delayed use of low-dose tacrolimus after aortic transplantation suppresses donor-specific anti-MHC class I and class II antibody production in rats / $c I. Matia, P. Fellmer, K. Splith, M. Varga, M. Adamec, I. Kämmerer, L. Feldbrügge, F. Krenzien, HM. Hau, G. Atanasov, M. Schmelzle, S. Jonas,
520    9_
$a BACKGROUND: Arterial allografts are used as vascular conduits in the treatment of prosthetic graft infection. Immunosuppression decreases their rupture risk rate. However, immunosuppression can be unprofitable in florid infection. Previously, we confirmed inhibition of cell-mediated destruction of rat aortic grafts by delayed use of tacrolimus. In this work, we studied the influence of this protocol on the antibody-mediated rejection. MATERIAL AND METHODS: Flow cytometry was used for the retrospective analysis of day 0, 14, and 30 sera obtained from Lewis rat recipients of isogeneic fresh infrarenal aortic grafts (group A) or Brown-Norway rat aortic grafts (group B,C,D) for the presence of donor-specific anti-MHC class I and II antibodies. Tacrolimus in daily dose of 0.2 mg/kg was administered from day 1 to day 30 (group C) or from day 7 to day 30 (group D). RESULTS: Inhibition of fluorescence-labeled anti-BN MHC class I and MHC class II antibodies binding to BN-splenocytes was observed only by day 14 and day 30 sera of allogeneic non-immunosuppressed Lewis rats (group B). The day 30 sera significantly decreased anti-MHC I (42±3%) and anti-MHC II antibody binding (56±3%) compared to day 0 (76±9%, p=0.005 and 79±5%, p=0.003, respectively). Deposition of immunoglobulins G into the tunica media was observed only in non-immunosuppressed aortic allografts on day 30. CONCLUSIONS: Fresh aortic allografts induce donor-specific anti-MHC class I and anti-MHC class II antibody production. Delayed administration of tacrolimus completely suppressed antibody production and antibody-mediated destruction of aortic allografts.
650    _2
$a zvířata $7 D000818
650    _2
$a aorta $x transplantace $7 D001011
650    _2
$a B-lymfocyty $x imunologie $7 D001402
650    _2
$a CD8-pozitivní T-lymfocyty $x imunologie $7 D018414
650    _2
$a vztah mezi dávkou a účinkem léčiva $7 D004305
650    _2
$a rejekce štěpu $x farmakoterapie $x imunologie $7 D006084
650    _2
$a MHC antigeny I. třídy $x imunologie $7 D015395
650    _2
$a MHC antigeny II. třídy $x imunologie $7 D000949
650    _2
$a imunosupresiva $x farmakologie $7 D007166
650    _2
$a isoprotilátky $x imunologie $7 D007518
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a potkani inbrední BN $7 D011914
650    _2
$a potkani inbrední LEW $7 D011917
650    _2
$a slezina $x cytologie $x imunologie $7 D013154
650    _2
$a takrolimus $x farmakologie $7 D016559
650    _2
$a homologní transplantace $7 D014184
650    _2
$a transplantace cév $x metody $7 D058017
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Fellmer, Peter $u Department of Visceral, Transplantation, Thoracic and Vascular Surgery, University of Leipzig, Leipzig, Germany.
700    1_
$a Splith, Katrin $u Translational Centre for Regenerative Medicine, University of Leipzig, Leipzig, Germany.
700    1_
$a Varga, Martin $u Department of Transplant Surgery, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
700    1_
$a Adamec, Milos $u Department of Transplant Surgery, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. $7 gn_A_00001257
700    1_
$a Kämmerer, Ines $u Translational Centre for Regenerative Medicine, University of Leipzig, Leipzig, Germany.
700    1_
$a Feldbrügge, Linda $u Department of Visceral, Transplantation, Thoracic and Vascular Surgery, University of Leipzig, Leipzig, Germany.
700    1_
$a Krenzien, Felix $u Department of Visceral, Transplantation, Thoracic and Vascular Surgery, University of Leipzig, Leipzig, Germany.
700    1_
$a Hau, Hans-Michael $u Department of Visceral, Transplantation, Thoracic and Vascular Surgery, University of Leipzig, Leipzig, Germany.
700    1_
$a Atanasov, Georgi $u Department of Visceral, Transplantation, Thoracic and Vascular Surgery, University of Leipzig, Leipzig, Germany. $7 gn_A_00009694
700    1_
$a Schmelzle, Moritz $u Department of Visceral, Transplantation, Thoracic and Vascular Surgery, University of Leipzig, Leipzig, Germany.
700    1_
$a Jonas, Sven $u Department of Visceral, Transplantation, Thoracic and Vascular Surgery, University of Leipzig, Leipzig, Germany.
773    0_
$w MED00000447 $t Annals of transplantation : quarterly of the Polish Transplantation Society $x 2329-0358 $g Roč. 19, č. - (2014), s. 225-32
856    41
$u https://pubmed.ncbi.nlm.nih.gov/24815872 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20141006 $b ABA008
991    __
$a 20141007102740 $b ABA008
999    __
$a ok $b bmc $g 1042096 $s 873125
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 19 $c - $d 225-32 $i 2329-0358 $m Annals of Transplantation $n Ann Transplant $x MED00000447
LZP    __
$a Pubmed-20141006
Back

Find record

Citation metrics

Archiving options