Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

MicroRNA profiling of activated and tolerogenic human dendritic cells

Z. Stumpfova, R. Hezova, AC. Meli, O. Slaby, J. Michalek,

. 2014 ; 2014 (-) : 259689.

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc15008066

Grantová podpora
NT13547 MZ0 CEP - Centrální evidence projektů

Dendritic cells (DCs) belong to the immune system and are particularly studied for their potential to direct either an activated or tolerogenic immune response. The roles of microRNAs (miRNAs) in posttranscriptional gene expression regulation are being increasingly investigated. This study's aim is to evaluate the miRNAs' expression changes in prepared human immature (iDCs), activated (aDCs), and tolerogenic dendritic cells (tDCs). The dendritic cells were prepared using GM-CSF and IL-4 (iDC) and subsequently maturated by adding LPS and IFN-γ (aDC) or IL-10 and TGF-β (tDC). Surface markers, cytokine profiles, and miRNA profiles were evaluated in iDC, tDC, and aDC at 6 h and 24 h of maturation. We identified 4 miRNAs (miR-7, miR-9, miR-155 and miR-182), which were consistently overexpressed in aDC after 6 h and 24 h of maturation and 3 miRNAs (miR-17, miR-133b, and miR-203) and miR-23b cluster solely expressed in tDC. We found 5 miRNAs (miR-10a, miR-203, miR-210, miR-30a, and miR-449b) upregulated and 3 miRNAs downregulated (miR-134, miR-145, and miR-149) in both tDC and aDC. These results indicate that miRNAs are specifically modulated in human DC types. This work may contribute to identifying specific modulating miRNAs for aDC and tDC, which could in the future serve as therapeutic targets in the treatment of cancer and autoimmune diseases.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc15008066
003      
CZ-PrNML
005      
20191014100152.0
007      
ta
008      
150306s2014 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1155/2014/259689 $2 doi
035    __
$a (PubMed)24799764
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Stumpfova, Zuzana $u Department of Pharmacology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
245    10
$a MicroRNA profiling of activated and tolerogenic human dendritic cells / $c Z. Stumpfova, R. Hezova, AC. Meli, O. Slaby, J. Michalek,
520    9_
$a Dendritic cells (DCs) belong to the immune system and are particularly studied for their potential to direct either an activated or tolerogenic immune response. The roles of microRNAs (miRNAs) in posttranscriptional gene expression regulation are being increasingly investigated. This study's aim is to evaluate the miRNAs' expression changes in prepared human immature (iDCs), activated (aDCs), and tolerogenic dendritic cells (tDCs). The dendritic cells were prepared using GM-CSF and IL-4 (iDC) and subsequently maturated by adding LPS and IFN-γ (aDC) or IL-10 and TGF-β (tDC). Surface markers, cytokine profiles, and miRNA profiles were evaluated in iDC, tDC, and aDC at 6 h and 24 h of maturation. We identified 4 miRNAs (miR-7, miR-9, miR-155 and miR-182), which were consistently overexpressed in aDC after 6 h and 24 h of maturation and 3 miRNAs (miR-17, miR-133b, and miR-203) and miR-23b cluster solely expressed in tDC. We found 5 miRNAs (miR-10a, miR-203, miR-210, miR-30a, and miR-449b) upregulated and 3 miRNAs downregulated (miR-134, miR-145, and miR-149) in both tDC and aDC. These results indicate that miRNAs are specifically modulated in human DC types. This work may contribute to identifying specific modulating miRNAs for aDC and tDC, which could in the future serve as therapeutic targets in the treatment of cancer and autoimmune diseases.
650    _2
$a kultivované buňky $7 D002478
650    _2
$a dendritické buňky $x účinky léků $x metabolismus $7 D003713
650    _2
$a průtoková cytometrie $7 D005434
650    _2
$a faktor stimulující granulocyto-makrofágové kolonie $x farmakologie $7 D016178
650    _2
$a lidé $7 D006801
650    _2
$a interleukin-10 $x farmakologie $7 D016753
650    _2
$a interleukin-4 $x farmakologie $7 D015847
650    _2
$a lipopolysacharidy $x farmakologie $7 D008070
650    _2
$a mikro RNA $x genetika $7 D035683
650    _2
$a transformující růstový faktor beta $x farmakologie $7 D016212
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Héžová, Renata $u CEITEC, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic ; Masaryk Memorial Cancer Institute, Zlutý Kopec 7, 65653 Brno, Czech Republic. $7 xx0157096
700    1_
$a Meli, Albano Carlo $u Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic ; INSERM U1046, University of Montpellier I and II, 371 avenue du Doyen G. Giraud, 34295 Montpellier, France.
700    1_
$a Slabý, Ondřej, $u CEITEC, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic ; Masaryk Memorial Cancer Institute, Zlutý Kopec 7, 65653 Brno, Czech Republic. $d 1981- $7 js20030220015
700    1_
$a Michalek, Jaroslav $u Department of Pharmacology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
773    0_
$w MED00006378 $t Mediators of inflammation $x 1466-1861 $g Roč. 2014, č. - (2014), s. 259689
856    41
$u https://pubmed.ncbi.nlm.nih.gov/24799764 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20150306 $b ABA008
991    __
$a 20191014100616 $b ABA008
999    __
$a ok $b bmc $g 1065339 $s 890866
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 2014 $c - $d 259689 $i 1466-1861 $m Mediators of inflammation $n Mediators Inflamm $x MED00006378
GRA    __
$a NT13547 $p MZ0
LZP    __
$a Pubmed-20150306

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace