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Quantification of potassium levels in cells treated with Bordetella adenylate cyclase toxin
T. Wald, I. Petry-Podgorska, R. Fiser, T. Matousek, J. Dedina, R. Osicka, P. Sebo, J. Masin,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
ProQuest Central
od 2013-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2003-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2013-01-01 do Před 1 rokem
PubMed
24412166
DOI
10.1016/j.ab.2013.10.039
Knihovny.cz E-zdroje
- MeSH
- adenylátcyklasový toxin toxicita MeSH
- antigeny CD11b genetika MeSH
- antigeny CD18 genetika MeSH
- Bordetella pertussis enzymologie MeSH
- CHO buňky MeSH
- Cricetulus MeSH
- draslík chemie metabolismus MeSH
- fluorescenční barviva chemie MeSH
- hmotnostní spektrometrie metody MeSH
- intracelulární prostor účinky léků metabolismus MeSH
- křečci praví MeSH
- lidé MeSH
- transfekce MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The aim of this study was to compare two methods for quantification of changes in intracellular potassium concentration (decrease from ∼140 to ∼20mM) due to the action of a pore-forming toxin, the adenylate cyclase toxin (CyaA) from the pathogenic bacterium Bordetella pertussis. CyaA was incubated with stably transfected K1 Chinese hamster ovary cells expressing the toxin receptor CD11b/CD18 and the decrease in potassium concentration in the cells was followed by inductively coupled plasma mass spectrometry (ICP-MS). It is shown that this method is superior in terms of sensitivity, accuracy, and temporal resolution over the method employing the potassium-binding benzofuran isophthalate-acetoxymethyl ester fluorescent indicator. The ICP-MS procedure was found to be a reliable and straightforward analytical approach enabling kinetic studies of CyaA action at physiologically relevant toxin concentrations (<1000ng/ml) in biological microsamples.
Citace poskytuje Crossref.org
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