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Characterization of tetratricopeptide repeat-like proteins in Francisella tularensis and identification of a novel locus required for virulence

V. Dankova, L. Balonova, A. Straskova, P. Spidlova, D. Putzova, T. Kijek, J. Bozue, C. Cote, S. Mou, P. Worsham, B. Szotakova, L. Cerveny, J. Stulik,

. 2014 ; 82 (12) : 5035-48.

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc15014060
E-zdroje Online Plný text

NLK Free Medical Journals od 1970 do Před 6 měsíci
Freely Accessible Science Journals od 1995 do Před 6 měsíci
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Open Access Digital Library od 1970-01-01

Odkazy

PubMed 25245806
DOI 10.1128/iai.01620-14
Knihovny.cz E-zdroje

Francisella tularensis is a highly infectious bacterium that causes the potentially lethal disease tularemia. This extremely virulent bacterium is able to replicate in the cytosolic compartments of infected macrophages. To invade macrophages and to cope with their intracellular environment, Francisella requires multiple virulence factors, which are still being identified. Proteins containing tetratricopeptide repeat (TPR)-like domains seem to be promising targets to investigate, since these proteins have been reported to be directly involved in virulence-associated functions of bacterial pathogens. Here, we studied the role of the FTS_0201, FTS_0778, and FTS_1680 genes, which encode putative TPR-like proteins in Francisella tularensis subsp. holarctica FSC200. Mutants defective in protein expression were prepared by TargeTron insertion mutagenesis. We found that the locus FTS_1680 and its ortholog FTT_0166c in the highly virulent Francisella tularensis type A strain SchuS4 are required for proper intracellular replication, full virulence in mice, and heat stress tolerance. Additionally, the FTS_1680-encoded protein was identified as a membrane-associated protein required for full cytopathogenicity in macrophages. Our study thus identifies FTS_1680/FTT_0166c as a new virulence factor in Francisella tularensis.

Citace poskytuje Crossref.org

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