-
Je něco špatně v tomto záznamu ?
Acinetobacter gandensis sp. nov. isolated from horse and cattle
A. Smet, P. Cools, L. Krizova, M. Maixnerova, O. Sedo, F. Haesebrouck, M. Kempf, A. Nemec, M. Vaneechoutte,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Free Medical Journals
od 1951 do Před 2 roky
Freely Accessible Science Journals
od 2000 do Před 1 rokem
PubMed
25225259
DOI
10.1099/ijs.0.068791-0
Knihovny.cz E-zdroje
- MeSH
- Acinetobacter klasifikace genetika izolace a purifikace MeSH
- bakteriální geny MeSH
- DNA bakterií genetika MeSH
- feces mikrobiologie MeSH
- fylogeneze * MeSH
- hybridizace nukleových kyselin MeSH
- koně mikrobiologie MeSH
- mastné kyseliny chemie MeSH
- molekulární sekvence - údaje MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- skot mikrobiologie MeSH
- techniky typizace bakterií MeSH
- zastoupení bazí MeSH
- zvířata MeSH
- Check Tag
- skot mikrobiologie MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Belgie MeSH
We previously reported the presence of an OXA-23 carbapenemase in an undescribed species of the genus Acinetobacter isolated from horse dung at the Faculty of Veterinary Medicine, Ghent University, Belgium. Here we include six strains to corroborate the delineation of this taxon by phenotypic characterization, DNA-DNA hybridization, 16S rRNA gene and rpoB sequence analysis, % G+C determination, MALDI-TOF MS and fatty acid analysis. The nearly complete 16S rRNA gene sequence of strain UG 60467(T) showed the highest similarities with those of the type strains of Acinetobacter bouvetii (98.4 %), Acinetobacter haemolyticus (97.7 %), and Acinetobacter schindleri (97.2 %). The partial rpoB sequence of strain UG 60467(T) showed the highest similarities with 'Acinetobacter bohemicus' ANC 3994 (88.6 %), A. bouvetii NIPH 2281 (88.6 %) and A. schindleri CIP 107287T (87.3 %). Whole-cell MALDI-TOF MS analyses supported the distinctness of the group at the protein level. The predominant fatty acids of strain UG 60467(T) were C12 : 0 3-OH, C12 : 0, C16 : 0, C18 : 1ω9c and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). Strains UG 60467(T) and UG 60716 showed a DNA-DNA relatedness of 84 % with each other and a DNA-DNA relatedness with A. schindleri LMG 19576(T) of 17 % and 20 %, respectively. The DNA G+C content of strain UG 60467(T) was 39.6 mol%. The name Acinetobacter gandensis sp. nov. is proposed for the novel taxon. The type strain is UG 60467(T) ( = ANC 4275(T) = LMG 27960(T) = DSM 28097(T)).
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15014071
- 003
- CZ-PrNML
- 005
- 20170719140959.0
- 007
- ta
- 008
- 150420s2014 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1099/ijs.0.068791-0 $2 doi
- 035 __
- $a (PubMed)25225259
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Smet, Annemieke $u Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan133, 9820 Merelbeke, Belgium.
- 245 10
- $a Acinetobacter gandensis sp. nov. isolated from horse and cattle / $c A. Smet, P. Cools, L. Krizova, M. Maixnerova, O. Sedo, F. Haesebrouck, M. Kempf, A. Nemec, M. Vaneechoutte,
- 520 9_
- $a We previously reported the presence of an OXA-23 carbapenemase in an undescribed species of the genus Acinetobacter isolated from horse dung at the Faculty of Veterinary Medicine, Ghent University, Belgium. Here we include six strains to corroborate the delineation of this taxon by phenotypic characterization, DNA-DNA hybridization, 16S rRNA gene and rpoB sequence analysis, % G+C determination, MALDI-TOF MS and fatty acid analysis. The nearly complete 16S rRNA gene sequence of strain UG 60467(T) showed the highest similarities with those of the type strains of Acinetobacter bouvetii (98.4 %), Acinetobacter haemolyticus (97.7 %), and Acinetobacter schindleri (97.2 %). The partial rpoB sequence of strain UG 60467(T) showed the highest similarities with 'Acinetobacter bohemicus' ANC 3994 (88.6 %), A. bouvetii NIPH 2281 (88.6 %) and A. schindleri CIP 107287T (87.3 %). Whole-cell MALDI-TOF MS analyses supported the distinctness of the group at the protein level. The predominant fatty acids of strain UG 60467(T) were C12 : 0 3-OH, C12 : 0, C16 : 0, C18 : 1ω9c and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). Strains UG 60467(T) and UG 60716 showed a DNA-DNA relatedness of 84 % with each other and a DNA-DNA relatedness with A. schindleri LMG 19576(T) of 17 % and 20 %, respectively. The DNA G+C content of strain UG 60467(T) was 39.6 mol%. The name Acinetobacter gandensis sp. nov. is proposed for the novel taxon. The type strain is UG 60467(T) ( = ANC 4275(T) = LMG 27960(T) = DSM 28097(T)).
- 650 _2
- $a Acinetobacter $x klasifikace $x genetika $x izolace a purifikace $7 D000150
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a techniky typizace bakterií $7 D015373
- 650 _2
- $a zastoupení bazí $7 D001482
- 650 _2
- $a skot $x mikrobiologie $7 D002417
- 650 _2
- $a DNA bakterií $x genetika $7 D004269
- 650 _2
- $a mastné kyseliny $x chemie $7 D005227
- 650 _2
- $a feces $x mikrobiologie $7 D005243
- 650 _2
- $a bakteriální geny $7 D005798
- 650 _2
- $a koně $x mikrobiologie $7 D006736
- 650 _2
- $a molekulární sekvence - údaje $7 D008969
- 650 _2
- $a hybridizace nukleových kyselin $7 D009693
- 650 12
- $a fylogeneze $7 D010802
- 650 _2
- $a RNA ribozomální 16S $x genetika $7 D012336
- 650 _2
- $a sekvenční analýza DNA $7 D017422
- 651 _2
- $a Belgie $7 D001530
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Cools, Piet $u Laboratory Bacteriology Research (LBR), Department of Clinical Chemistry, Microbiology & Immunology, Ghent University Hospital, De Pintelaan 185, 9000 Gent, Belgium.
- 700 1_
- $a Krizova, Lenka $u Laboratory of Bacterial Genetics, National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic.
- 700 1_
- $a Maixnerová, Martina $7 xx0093284 $u Laboratory of Bacterial Genetics, National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic.
- 700 1_
- $a Sedo, Ondrej $u Research Group Proteomics, Central European Institute of Technology and National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
- 700 1_
- $a Haesebrouck, Freddy $u Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan133, 9820 Merelbeke, Belgium.
- 700 1_
- $a Kempf, Marie $u Laboratoire de Bactériologie, Institut de Biologie en Santé - PBU, CHU, 4 rue Larrey, 49933 Angers cedex, France.
- 700 1_
- $a Nemec, Alexandr $u Laboratory of Bacterial Genetics, National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic.
- 700 1_
- $a Vaneechoutte, Mario $u Laboratory Bacteriology Research (LBR), Department of Clinical Chemistry, Microbiology & Immunology, Ghent University Hospital, De Pintelaan 185, 9000 Gent, Belgium Mario.Vaneechoutte@UGent.be.
- 773 0_
- $w MED00006002 $t International journal of systematic and evolutionary microbiology $x 1466-5034 $g Roč. 64, č. Pt 12 (2014), s. 4007-15
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/25225259 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20150420 $b ABA008
- 991 __
- $a 20170719141451 $b ABA008
- 999 __
- $a ok $b bmc $g 1071652 $s 896949
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2014 $b 64 $c Pt 12 $d 4007-15 $i 1466-5034 $m International journal of systematic and evolutionary microbiology $n Int. j. syst. evol. microbiol. (Print) $x MED00006002
- LZP __
- $a Pubmed-20150420
