-
Something wrong with this record ?
The effect of Leonurus cardiaca herb extract and some of its flavonoids on mitochondrial oxidative phosphorylation in the heart
J. Bernatoniene, DM. Kopustinskiene, V. Jakstas, D. Majiene, R. Baniene, L. Kuršvietiene, R. Masteikova, A. Savickas, A. Toleikis, S. Trumbeckaite,
Language English Country Germany
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Antioxidants chemistry isolation & purification pharmacology MeSH
- Cell Respiration drug effects MeSH
- Flavonoids chemistry isolation & purification pharmacology MeSH
- Myocytes, Cardiac drug effects MeSH
- Rats MeSH
- Plants, Medicinal MeSH
- Plant Components, Aerial chemistry MeSH
- Oxidative Phosphorylation drug effects MeSH
- Hydrogen Peroxide metabolism MeSH
- Polyphenols chemistry isolation & purification pharmacology MeSH
- Rats, Wistar MeSH
- Plant Extracts chemistry isolation & purification pharmacology MeSH
- Mitochondria, Heart drug effects MeSH
- Leonurus chemistry MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Motherwort (Leonurus cardiaca) possesses antibacterial, antioxidant, anti-inflammatory, and analgesic activities, and is used as a complementary remedy to improve heart function and blood circulation. Since cardiovascular diseases are often associated with an alteration of mitochondria, the main producers of ATP in cardiac muscle cells, the aim of our work was to determine bioactive constituents present in motherwort aerial parts extract in ethanol and investigate their effects on the functions of cardiac mitochondria. Quantitative determination of polyphenols in L. cardiaca herb extract was performed by HPLC. Mitochondrial respiration rates were evaluated using a Clark-type oxygen electrode. Mitochondrial ROS generation was determined fluorimetrically with Amplex Red and horseradish peroxidase. The results showed that constituents (chlorogenic acid, orientin, quercetin, hyperoside, and rutin) of L. cardiaca herb extract uncouple (by 20-90 %) mitochondrial oxidation from phosphorylation, partially inhibit (by ~ 40 %) the mitochondrial respiratory chain in cases of pyruvate and malate as well as succinate oxidation, and effectively attenuate the generation of free radicals in mitochondria. Since partial uncoupling of mitochondria, respiratory inhibition, and decreased ROS production are proposed as possible mechanisms of cardioprotection, our results imply that L. cardiaca herb extract could be a useful remedy to protect cardiac muscles from the effects of pathogenic processes.
Department of Biochemistry Medical Academy Lithuanian University of Health Sciences Kaunas Lithuania
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15014301
- 003
- CZ-PrNML
- 005
- 20150428124422.0
- 007
- ta
- 008
- 150420s2014 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1055/s-0034-1368426 $2 doi
- 035 __
- $a (PubMed)24841965
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Bernatoniene, Jurga $u Department of Drug Technology and Social Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
- 245 14
- $a The effect of Leonurus cardiaca herb extract and some of its flavonoids on mitochondrial oxidative phosphorylation in the heart / $c J. Bernatoniene, DM. Kopustinskiene, V. Jakstas, D. Majiene, R. Baniene, L. Kuršvietiene, R. Masteikova, A. Savickas, A. Toleikis, S. Trumbeckaite,
- 520 9_
- $a Motherwort (Leonurus cardiaca) possesses antibacterial, antioxidant, anti-inflammatory, and analgesic activities, and is used as a complementary remedy to improve heart function and blood circulation. Since cardiovascular diseases are often associated with an alteration of mitochondria, the main producers of ATP in cardiac muscle cells, the aim of our work was to determine bioactive constituents present in motherwort aerial parts extract in ethanol and investigate their effects on the functions of cardiac mitochondria. Quantitative determination of polyphenols in L. cardiaca herb extract was performed by HPLC. Mitochondrial respiration rates were evaluated using a Clark-type oxygen electrode. Mitochondrial ROS generation was determined fluorimetrically with Amplex Red and horseradish peroxidase. The results showed that constituents (chlorogenic acid, orientin, quercetin, hyperoside, and rutin) of L. cardiaca herb extract uncouple (by 20-90 %) mitochondrial oxidation from phosphorylation, partially inhibit (by ~ 40 %) the mitochondrial respiratory chain in cases of pyruvate and malate as well as succinate oxidation, and effectively attenuate the generation of free radicals in mitochondria. Since partial uncoupling of mitochondria, respiratory inhibition, and decreased ROS production are proposed as possible mechanisms of cardioprotection, our results imply that L. cardiaca herb extract could be a useful remedy to protect cardiac muscles from the effects of pathogenic processes.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a antioxidancia $x chemie $x izolace a purifikace $x farmakologie $7 D000975
- 650 _2
- $a buněčné dýchání $x účinky léků $7 D019069
- 650 _2
- $a flavonoidy $x chemie $x izolace a purifikace $x farmakologie $7 D005419
- 650 _2
- $a peroxid vodíku $x metabolismus $7 D006861
- 650 _2
- $a srdečník $x chemie $7 D031332
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a srdeční mitochondrie $x účinky léků $7 D008929
- 650 _2
- $a kardiomyocyty $x účinky léků $7 D032383
- 650 _2
- $a oxidativní fosforylace $x účinky léků $7 D010085
- 650 _2
- $a nadzemní části rostlin $x chemie $7 D035261
- 650 _2
- $a rostlinné extrakty $x chemie $x izolace a purifikace $x farmakologie $7 D010936
- 650 _2
- $a léčivé rostliny $7 D010946
- 650 _2
- $a polyfenoly $x chemie $x izolace a purifikace $x farmakologie $7 D059808
- 650 _2
- $a krysa rodu Rattus $7 D051381
- 650 _2
- $a potkani Wistar $7 D017208
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Kopustinskiene, Dalia M $u Department of Drug Technology and Social Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
- 700 1_
- $a Jakstas, Valdas $u Department of Pharmacognosy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
- 700 1_
- $a Majiene, Daiva $u Department of Drug Technology and Social Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
- 700 1_
- $a Baniene, Rasa $u Laboratory of Biochemistry, Neuroscience Institute of Lithuanian University of Health Sciences, Kaunas, Lithuania.
- 700 1_
- $a Kuršvietiene, Lolita $u Department of Biochemistry, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
- 700 1_
- $a Masteikova, Ruta $u University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Pharmacy, Department of Pharmaceutics, Brno, Czech Republic.
- 700 1_
- $a Savickas, Arunas $u Department of Drug Technology and Social Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
- 700 1_
- $a Toleikis, Adolfas $u Laboratory of Biochemistry, Neuroscience Institute of Lithuanian University of Health Sciences, Kaunas, Lithuania.
- 700 1_
- $a Trumbeckaite, Sonata $u Laboratory of Biochemistry, Neuroscience Institute of Lithuanian University of Health Sciences, Kaunas, Lithuania.
- 773 0_
- $w MED00003839 $t Planta medica $x 1439-0221 $g Roč. 80, č. 7 (2014), s. 525-32
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/24841965 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20150420 $b ABA008
- 991 __
- $a 20150428124726 $b ABA008
- 999 __
- $a ok $b bmc $g 1071882 $s 897179
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2014 $b 80 $c 7 $d 525-32 $i 1439-0221 $m Planta medica $n Planta Med $x MED00003839
- LZP __
- $a Pubmed-20150420
