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Defensin from the ornate sheep tick Dermacentor marginatus and its effect on Lyme borreliosis spirochetes
T. Chrudimská, V. Čeřovský, J. Slaninová, RO. Rego, L. Grubhoffer,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anti-Bacterial Agents isolation & purification pharmacology MeSH
- Antifungal Agents isolation & purification pharmacology MeSH
- Antiviral Agents isolation & purification pharmacology MeSH
- Borrelia burgdorferi Group drug effects MeSH
- Defensins isolation & purification pharmacology MeSH
- Dermacentor chemistry MeSH
- Microbial Sensitivity Tests MeSH
- Arthropod Proteins isolation & purification pharmacology MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Expression of the previously reported defensin of the tick Dermacentor marginatus (defDM) was analysed in different organs by RT-PCR. mRNA of the defDM gene was detected in the hemolymph, midgut and salivary glands. Moreover defDM was isolated from the tick hemolymph using RP-HPLC and its sequence was determined by mass spectrometry and Edman degradation. Synthetic peptide was used for determining biological activities. The results showed an anti-Gram-positive bacterial role for the defensin. As D. marginatus ticks appear not to be vectors of the Lyme disease agent of the complex Borrelia burgdorferi sensu lato, we tested the influence of defDM on Borrelia afzelii. There is a very clear borrelicidal activity of the defensin, which is concentration dependent and suggests a possible role in the clearing of Borrelia ingested by D. marginatus ticks.
University of South Bohemia Faculty of Science Branišovská 31 370 05 České Budějovice Czech Republic
References provided by Crossref.org
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