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Defensin from the ornate sheep tick Dermacentor marginatus and its effect on Lyme borreliosis spirochetes
T. Chrudimská, V. Čeřovský, J. Slaninová, RO. Rego, L. Grubhoffer,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- antibakteriální látky izolace a purifikace farmakologie MeSH
- antifungální látky izolace a purifikace farmakologie MeSH
- antivirové látky izolace a purifikace farmakologie MeSH
- Borrelia burgdorferi komplex účinky léků MeSH
- defensiny izolace a purifikace farmakologie MeSH
- Dermacentor chemie MeSH
- mikrobiální testy citlivosti MeSH
- proteiny členovců izolace a purifikace farmakologie MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Expression of the previously reported defensin of the tick Dermacentor marginatus (defDM) was analysed in different organs by RT-PCR. mRNA of the defDM gene was detected in the hemolymph, midgut and salivary glands. Moreover defDM was isolated from the tick hemolymph using RP-HPLC and its sequence was determined by mass spectrometry and Edman degradation. Synthetic peptide was used for determining biological activities. The results showed an anti-Gram-positive bacterial role for the defensin. As D. marginatus ticks appear not to be vectors of the Lyme disease agent of the complex Borrelia burgdorferi sensu lato, we tested the influence of defDM on Borrelia afzelii. There is a very clear borrelicidal activity of the defensin, which is concentration dependent and suggests a possible role in the clearing of Borrelia ingested by D. marginatus ticks.
University of South Bohemia Faculty of Science Branišovská 31 370 05 České Budějovice Czech Republic
Citace poskytuje Crossref.org
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