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Validácia stanovenia 1-hydroxypyrénu v moči metódou HPLC
[Validation of HPLC determination of 1-hydroxypyrene in urine]
Ivica Bajusová, Ľubomír Legáth
Language Slovak Country Czech Republic
- MeSH
- Biomarkers * analysis chemistry MeSH
- Chemistry Techniques, Analytical MeSH
- Microscopy, Fluorescence methods utilization MeSH
- Humans MeSH
- Biomarkers, Tumor analysis chemistry MeSH
- Polycyclic Aromatic Hydrocarbons * analysis metabolism urine adverse effects MeSH
- Pyrenes * analysis urine MeSH
- Chromatography, High Pressure Liquid * methods utilization MeSH
- Environmental Exposure analysis MeSH
- Check Tag
- Humans MeSH
Exposure to polycyclic aromatic hydrocarbons (PAH) is a serious hazard for human health. 1-Hydroxypyrene (1-HOP), a major metabolite of pyrene, is used as a biomarker for the assessment of an exposure to PAH. The article deals with the validation of the HPLC determination of 1-HOP with fluorescence detection. 1-HOP was isolated from urine by solid phase extraction. The detection and quantification limits are 0.08 and 0.26 g l–1, respectively. RSD of precision and accuracy are lower than 9.8 %. The recoveries are 78.8–93.5 %. This method can be used to determine and monitor the urinary 1-HOP in workers exposed to PAH.
Validation of HPLC determination of 1-hydroxypyrene in urine
Literatura
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- $a Bajusová, Ivica $7 xx0325755 $u Klinika pracovného lekárstva a klinickej toxikológie Lekárskej fakulty Univerzity P. J. Šafárika Košice a Univerzitnej nemocnice L. Pasteura Košice
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- $a Validácia stanovenia 1-hydroxypyrénu v moči metódou HPLC / $c Ivica Bajusová, Ľubomír Legáth
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- $a Validation of HPLC determination of 1-hydroxypyrene in urine
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- $a Literatura
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- $a Exposure to polycyclic aromatic hydrocarbons (PAH) is a serious hazard for human health. 1-Hydroxypyrene (1-HOP), a major metabolite of pyrene, is used as a biomarker for the assessment of an exposure to PAH. The article deals with the validation of the HPLC determination of 1-HOP with fluorescence detection. 1-HOP was isolated from urine by solid phase extraction. The detection and quantification limits are 0.08 and 0.26 g l–1, respectively. RSD of precision and accuracy are lower than 9.8 %. The recoveries are 78.8–93.5 %. This method can be used to determine and monitor the urinary 1-HOP in workers exposed to PAH.
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- $a Legáth, Ľubomír $7 xx0074096 $u Klinika pracovného lekárstva a klinickej toxikológie Lekárskej fakulty Univerzity P. J. Šafárika Košice a Univerzitnej nemocnice L. Pasteura Košice
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- $t Chemické listy $x 0009-2770 $g Roč. 109, č. 1 (2015), s. 55-60 $w MED00011010
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- $u http://www.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/415/415 $y domovská stránka časopisu
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