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Metody stanovení aktivity proteasomu a jejich aplikace
[Methods for determination of proteasome activity and their applications]
Jindřich Sedláček, Michaela Švécarová
Language Czech Country Czech Republic
Document type Research Support, Non-U.S. Gov't
- MeSH
- Spectrometry, Fluorescence methods utilization MeSH
- Humans MeSH
- Medication Therapy Management MeSH
- Protein Processing, Post-Translational MeSH
- Proteasome Endopeptidase Complex * analysis MeSH
- Drug Screening Assays, Antitumor MeSH
- Unfolded Protein Response MeSH
- Ubiquitination MeSH
- Ubiquitinated Proteins * analysis MeSH
- Check Tag
- Humans MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
The proteasome is a promising therapeutic target in the treatment of malignant diseases. Inasmuch as cancer cells are characterized by a rapid synthesis of some proteins, which must be subsequently degraded. A brief description of and some methods for determination of enzymatic activity of proteasomes are given. The methods are used in kinetics studies of proteasome and in the screening of potentially active compounds. Future progress in development of these methods can contribute to more detailed studies of the pathological status of cells as well as to effective pharmacological regimens for patients treated with proteasome inhibitors.
Methods for determination of proteasome activity and their applications
Literatura
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