Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Influence of Trichobilharzia regenti (Digenea: Schistosomatidae) on the defence activity of Radix lagotis (Lymnaeidae) Haemocytes

V. Skála, A. Černíková, Z. Jindrová, M. Kašný, M. Vostrý, AJ. Walker, P. Horák,

. 2014 ; 9 (11) : e111696.

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc15022940

Radix lagotis is an intermediate snail host of the nasal bird schistosome Trichobilharzia regenti. Changes in defence responses in infected snails that might be related to host-parasite compatibility are not known. This study therefore aimed to characterize R. lagotis haemocyte defence mechanisms and determine the extent to which they are modulated by T. regenti. Histological observations of R. lagotis infected with T. regenti revealed that early phases of infection were accompanied by haemocyte accumulation around the developing larvae 2-36 h post exposure (p.e.) to the parasite. At later time points, 44-92 h p.e., no haemocytes were observed around T. regenti. Additionally, microtubular aggregates likely corresponding to phagocytosed ciliary plates of T. regenti miracidia were observed within haemocytes by use of transmission electron microscopy. When the infection was in the patent phase, haemocyte phagocytic activity and hydrogen peroxide production were significantly reduced in infected R. lagotis when compared to uninfected counterparts, whereas haemocyte abundance increased in infected snails. At a molecular level, protein kinase C (PKC) and extracellular-signal regulated kinase (ERK) were found to play an important role in regulating these defence reactions in R. lagotis. Moreover, haemocytes from snails with patent infection displayed lower PKC and ERK activity in cell adhesion assays when compared to those from uninfected snails, which may therefore be related to the reduced defence activities of these cells. These data provide the first integrated insight into the immunobiology of R. lagotis and demonstrate modulation of haemocyte-mediated responses in patent T. regenti infected snails. Given that immunomodulation occurs during patency, interference of snail-host defence by T. regenti might be important for the sustained production and/or release of infective cercariae.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc15022940
003      
CZ-PrNML
005      
20240903091216.0
007      
ta
008      
150709s2014 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1371/journal.pone.0111696 $2 doi
035    __
$a (PubMed)25372492
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Skála, Vladimír $u Charles University in Prague, Faculty of Science, Department of Parasitology, Prague, Czech Republic.
245    10
$a Influence of Trichobilharzia regenti (Digenea: Schistosomatidae) on the defence activity of Radix lagotis (Lymnaeidae) Haemocytes / $c V. Skála, A. Černíková, Z. Jindrová, M. Kašný, M. Vostrý, AJ. Walker, P. Horák,
520    9_
$a Radix lagotis is an intermediate snail host of the nasal bird schistosome Trichobilharzia regenti. Changes in defence responses in infected snails that might be related to host-parasite compatibility are not known. This study therefore aimed to characterize R. lagotis haemocyte defence mechanisms and determine the extent to which they are modulated by T. regenti. Histological observations of R. lagotis infected with T. regenti revealed that early phases of infection were accompanied by haemocyte accumulation around the developing larvae 2-36 h post exposure (p.e.) to the parasite. At later time points, 44-92 h p.e., no haemocytes were observed around T. regenti. Additionally, microtubular aggregates likely corresponding to phagocytosed ciliary plates of T. regenti miracidia were observed within haemocytes by use of transmission electron microscopy. When the infection was in the patent phase, haemocyte phagocytic activity and hydrogen peroxide production were significantly reduced in infected R. lagotis when compared to uninfected counterparts, whereas haemocyte abundance increased in infected snails. At a molecular level, protein kinase C (PKC) and extracellular-signal regulated kinase (ERK) were found to play an important role in regulating these defence reactions in R. lagotis. Moreover, haemocytes from snails with patent infection displayed lower PKC and ERK activity in cell adhesion assays when compared to those from uninfected snails, which may therefore be related to the reduced defence activities of these cells. These data provide the first integrated insight into the immunobiology of R. lagotis and demonstrate modulation of haemocyte-mediated responses in patent T. regenti infected snails. Given that immunomodulation occurs during patency, interference of snail-host defence by T. regenti might be important for the sustained production and/or release of infective cercariae.
650    _2
$a zvířata $7 D000818
650    _2
$a extracelulárním signálem regulované MAP kinasy $x metabolismus $7 D048049
650    _2
$a hemocyty $x imunologie $x metabolismus $x parazitologie $7 D006434
650    _2
$a interakce hostitele a parazita $x imunologie $7 D006790
650    _2
$a peroxid vodíku $x metabolismus $7 D006861
650    _2
$a Lymnaea $x imunologie $x metabolismus $x parazitologie $7 D008195
650    _2
$a fagocytóza $x imunologie $7 D010587
650    _2
$a proteinkinasa C $x metabolismus $7 D011493
650    12
$a Schistosomatidae $x ultrastruktura $7 D012551
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Černíková, Alena $u Charles University in Prague, Faculty of Science, Institute of Applied Mathematics and Information Technologies, Prague, Czech Republic.
700    1_
$a Jindrová, Zuzana $u Charles University in Prague, Faculty of Science, Department of Parasitology, Prague, Czech Republic; Charles University in Prague, 1st Faculty of Medicine, Institute of Immunology and Microbiology, Prague, Czech Republic.
700    1_
$a Kašný, Martin $u Charles University in Prague, Faculty of Science, Department of Parasitology, Prague, Czech Republic; Masaryk University, Faculty of Science, Department of Botany and Zoology, Brno, Czech Republic.
700    1_
$a Vostrý, Martin $u Charles University in Prague, Faculty of Science, Department of Parasitology, Prague, Czech Republic; Institute of Haematology and Blood Transfusion, Prague, Czech Republic. $7 xx0321711
700    1_
$a Walker, Anthony J $u Molecular Parasitology Laboratory, School of Life Sciences, Kingston University, Kingston upon Thames, Surrey, United Kingdom.
700    1_
$a Horák, Petr $u Charles University in Prague, Faculty of Science, Department of Parasitology, Prague, Czech Republic.
773    0_
$w MED00180950 $t PloS one $x 1932-6203 $g Roč. 9, č. 11 (2014), s. e111696
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25372492 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20150709 $b ABA008
991    __
$a 20240903091213 $b ABA008
999    __
$a ok $b bmc $g 1083279 $s 905933
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2014 $b 9 $c 11 $d e111696 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
LZP    __
$a Pubmed-20150709

Find record

Citation metrics

Archiving options