-
Something wrong with this record ?
Fast ibuprofen, ketoprofen and naproxen simultaneous determination in human serum for clinical toxicology by GC-FID
T. Hložek, M. Bursová, R. Cabala,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't, Validation Study
- MeSH
- Chromatography, Gas methods MeSH
- Ibuprofen blood MeSH
- Ketoprofen blood MeSH
- Humans MeSH
- Naproxen blood MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Validation Study MeSH
OBJECTIVES: The aim of this study was to develop and validate a gas chromatographic method with flame ionization detection (GC-FID) for the measurement of ibuprofen, naproxen and ketoprofen for clinical toxicology purposes. DESIGN AND METHODS: 100μL of plasma was treated with methyl chloroformate and derivatized analytes were extracted with hexane. Optimal conditions of the derivatization procedure have been found using the experimental chemometric design (face-centered central composite design). The selectivity and efficiency of the procedure was confirmed by GC-MS. RESULTS: The assay was linear in the concentration range of 10-400μgmL(-1), with adequate accuracy and precision for GC-FID (98-106.7%, CV≤9.1%, respectively) and for GC-MS (99.3-105.5%, CV≤9.2%, respectively). CONCLUSION: The entire sample preparation procedure is completed within 5 min and the quantitative results are available within 35 min. The method was successfully applied to quantify the selected compounds in serum of patients from emergency units.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15023271
- 003
- CZ-PrNML
- 005
- 20150730101250.0
- 007
- ta
- 008
- 150709s2014 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.clinbiochem.2014.06.076 $2 doi
- 035 __
- $a (PubMed)25003797
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Hložek, Tomáš $u Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic. Electronic address: tomas.hlozek@vfn.cz.
- 245 10
- $a Fast ibuprofen, ketoprofen and naproxen simultaneous determination in human serum for clinical toxicology by GC-FID / $c T. Hložek, M. Bursová, R. Cabala,
- 520 9_
- $a OBJECTIVES: The aim of this study was to develop and validate a gas chromatographic method with flame ionization detection (GC-FID) for the measurement of ibuprofen, naproxen and ketoprofen for clinical toxicology purposes. DESIGN AND METHODS: 100μL of plasma was treated with methyl chloroformate and derivatized analytes were extracted with hexane. Optimal conditions of the derivatization procedure have been found using the experimental chemometric design (face-centered central composite design). The selectivity and efficiency of the procedure was confirmed by GC-MS. RESULTS: The assay was linear in the concentration range of 10-400μgmL(-1), with adequate accuracy and precision for GC-FID (98-106.7%, CV≤9.1%, respectively) and for GC-MS (99.3-105.5%, CV≤9.2%, respectively). CONCLUSION: The entire sample preparation procedure is completed within 5 min and the quantitative results are available within 35 min. The method was successfully applied to quantify the selected compounds in serum of patients from emergency units.
- 650 _2
- $a chromatografie plynová $x metody $7 D002849
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a ibuprofen $x krev $7 D007052
- 650 _2
- $a ketoprofen $x krev $7 D007660
- 650 _2
- $a naproxen $x krev $7 D009288
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 655 _2
- $a validační studie $7 D023361
- 700 1_
- $a Bursová, Miroslava $u Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
- 700 1_
- $a Cabala, Radomír $u Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
- 773 0_
- $w MED00001119 $t Clinical biochemistry $x 1873-2933 $g Roč. 47, č. 15 (2014), s. 109-11
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/25003797 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20150709 $b ABA008
- 991 __
- $a 20150730101338 $b ABA008
- 999 __
- $a ok $b bmc $g 1083609 $s 906264
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2014 $b 47 $c 15 $d 109-11 $i 1873-2933 $m Clinical biochemistry $n Clin Biochem $x MED00001119
- LZP __
- $a Pubmed-20150709
