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Label-free and amplification-free miR-124 detection in human cells
K. Smerkova, K. Hudcova, V. Vlahova, M. Vaculovicova, V. Pekarik, M. Masarik, V. Adam, R. Kizek,
Language English Country Greece
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 2006 to 1 year ago
ProQuest Central
from 2012-01-01
Medline Complete (EBSCOhost)
from 2014-06-01
Nursing & Allied Health Database (ProQuest)
from 2012-01-01
Health & Medicine (ProQuest)
from 2012-01-01
PubMed
25405852
DOI
10.3892/ijo.2014.2756
Knihovny.cz E-resources
- MeSH
- Biosensing Techniques * MeSH
- Humans MeSH
- Limit of Detection MeSH
- RNA, Messenger biosynthesis genetics MeSH
- MicroRNAs genetics isolation & purification MeSH
- Cell Line, Tumor MeSH
- Gene Expression Regulation, Neoplastic MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
MicroRNAs (miRNAs) are becoming a very important group of molecules especially since their connection to numerous diseases has been revealed. The potential in gene therapy as well as in diagnostics is being widely investigated leading to the demand of sensitive, selective and simple methods of isolation and detection. The combined advantages of magnetic particle-based separation with sensitive electrochemical detection may offer a very valuable tool for these purposes. In this study, the miR‑124 was targeted as an example analyte for development and optimization of the isolation procedure coupled to the electrochemical detection. The sensitivity of the method was demonstrated by the limit of detection at the level of nanomolar concentration (4 nM). To verify the applicability of the procedure to the real samples, miR‑124 was isolated from the human embryonic kidney cells naturally expressing this miRNA molecule and the results were compared to the amount of miR‑124 isolated from the cells transfected by the pENTR-miR‑124 plasmid leading to the overexpression of miR‑124.
Central European Institute of Technology Brno University of Technology CZ 616 00 Brno Czech Republic
References provided by Crossref.org
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