-
Something wrong with this record ?
Marine Isolates of Trimastix marina Form a Plesiomorphic Deep-branching Lineage within Preaxostyla, Separate from Other Known Trimastigids (Paratrimastix n. gen.)
Q. Zhang, P. Táborský, JD. Silberman, T. Pánek, I. Čepička, AG. Simpson,
Language English Country Germany
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Species Specificity MeSH
- Eukaryota classification genetics isolation & purification ultrastructure MeSH
- Flagella ultrastructure MeSH
- Phylogeny * MeSH
- Organelles ultrastructure MeSH
- RNA, Ribosomal, 18S genetics MeSH
- Aquatic Organisms classification genetics isolation & purification ultrastructure MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Trimastigids are free-living, anaerobic protists that are closely related to the symbiotic oxymonads, forming together the taxon Preaxostyla (Excavata: Metamonada). We isolated fourteen new strains morphologically corresponding to two species assigned to Trimastix (until now the only genus of trimastigids), Trimastix marina and Trimastix pyriformis. Unexpectedly, marine strains of Trimastix marina branch separately from freshwater strains of this morphospecies in SSU rRNA gene trees, and instead form the sister group of all other Preaxostyla. This position is confirmed by three-gene phylogenies. Ultrastructural examination of a marine isolate of Trimastix marina demonstrates a combination of trimastigid-like features (e.g. preaxostyle-like I fibre) and ancestral characters (e.g. absence of thickened flagellar vane margins), consistent with inclusion of marine T. marina within Preaxostyla, but also supporting its distinctiveness from 'freshwater T. marina' and its deep-branching position within Preaxostyla. Since these results indicate paraphyly of Trimastix as currently understood, we transfer the other better-studied trimastigids to Paratrimastix n. gen. and Paratrimastigidae n. fam. The freshwater form previously identified as T. marina is described as Paratrimastix eleionoma n. sp., and Trimastix pyriformis becomes Paratrimastix pyriformis n. comb. Because of its phylogenetic position, 'true' Trimastix is potentially important for understanding the evolution of mitochondrion-related organelles in metamonads.
Department of Biological Sciences University of Arkansas Fayetteville AR 72701 USA
Department of Biology Dalhousie University Halifax B3H 4R2 Canada
Yantai Institute of Coastal Zone Research Chinese Academy of Science Yantai 264003 China
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16000047
- 003
- CZ-PrNML
- 005
- 20160127103657.0
- 007
- ta
- 008
- 160108s2015 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.protis.2015.07.003 $2 doi
- 035 __
- $a (PubMed)26312987
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Zhang, Qianqian $u Department of Biology, Dalhousie University, Halifax, B3H 4R2, Canada; Yantai Institute of Coastal Zone Research, Chinese Academy of Science, Yantai 264003, China.
- 245 10
- $a Marine Isolates of Trimastix marina Form a Plesiomorphic Deep-branching Lineage within Preaxostyla, Separate from Other Known Trimastigids (Paratrimastix n. gen.) / $c Q. Zhang, P. Táborský, JD. Silberman, T. Pánek, I. Čepička, AG. Simpson,
- 520 9_
- $a Trimastigids are free-living, anaerobic protists that are closely related to the symbiotic oxymonads, forming together the taxon Preaxostyla (Excavata: Metamonada). We isolated fourteen new strains morphologically corresponding to two species assigned to Trimastix (until now the only genus of trimastigids), Trimastix marina and Trimastix pyriformis. Unexpectedly, marine strains of Trimastix marina branch separately from freshwater strains of this morphospecies in SSU rRNA gene trees, and instead form the sister group of all other Preaxostyla. This position is confirmed by three-gene phylogenies. Ultrastructural examination of a marine isolate of Trimastix marina demonstrates a combination of trimastigid-like features (e.g. preaxostyle-like I fibre) and ancestral characters (e.g. absence of thickened flagellar vane margins), consistent with inclusion of marine T. marina within Preaxostyla, but also supporting its distinctiveness from 'freshwater T. marina' and its deep-branching position within Preaxostyla. Since these results indicate paraphyly of Trimastix as currently understood, we transfer the other better-studied trimastigids to Paratrimastix n. gen. and Paratrimastigidae n. fam. The freshwater form previously identified as T. marina is described as Paratrimastix eleionoma n. sp., and Trimastix pyriformis becomes Paratrimastix pyriformis n. comb. Because of its phylogenetic position, 'true' Trimastix is potentially important for understanding the evolution of mitochondrion-related organelles in metamonads.
- 650 _2
- $a vodní organismy $x klasifikace $x genetika $x izolace a purifikace $x ultrastruktura $7 D059001
- 650 _2
- $a Eukaryota $x klasifikace $x genetika $x izolace a purifikace $x ultrastruktura $7 D056890
- 650 _2
- $a flagella $x ultrastruktura $7 D005407
- 650 _2
- $a organely $x ultrastruktura $7 D015388
- 650 12
- $a fylogeneze $7 D010802
- 650 _2
- $a RNA ribozomální 18S $x genetika $7 D012337
- 650 _2
- $a druhová specificita $7 D013045
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Táborský, Petr $u Department of Zoology, Faculty of Science, Charles University in Prague, Vinicna 7, 128 44 Prague 2, Czech Republic.
- 700 1_
- $a Silberman, Jeffrey D $u Department of Biological Sciences, University of Arkansas, Fayetteville, AR, 72701, USA.
- 700 1_
- $a Pánek, Tomáš $u Department of Zoology, Faculty of Science, Charles University in Prague, Vinicna 7, 128 44 Prague 2, Czech Republic.
- 700 1_
- $a Čepička, Ivan $u Department of Zoology, Faculty of Science, Charles University in Prague, Vinicna 7, 128 44 Prague 2, Czech Republic.
- 700 1_
- $a Simpson, Alastair G B $u Department of Biology, Dalhousie University, Halifax, B3H 4R2, Canada. Electronic address: asimpso2@dal.ca.
- 773 0_
- $w MED00005138 $t Protist $x 1618-0941 $g Roč. 166, č. 4 (2015), s. 468-91
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/26312987 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20160108 $b ABA008
- 991 __
- $a 20160127103821 $b ABA008
- 999 __
- $a ok $b bmc $g 1102328 $s 924253
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 166 $c 4 $d 468-91 $e 20150722 $i 1618-0941 $m Protist $n Protist (Jena, Print) $x MED00005138
- LZP __
- $a Pubmed-20160108
