• Je něco špatně v tomto záznamu ?

Antineoplastic effects of Chlorella pyrenoidosa in the breast cancer model

P. Kubatka, A. Kapinová, P. Kružliak, M. Kello, D. Výbohová, K. Kajo, M. Novák, M. Chripková, M. Adamkov, M. Péč, J. Mojžiš, B. Bojková, M. Kassayová, N. Stollárová, D. Dobrota,

. 2015 ; 31 (4) : 560-9. [pub] 20140906

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16000214
E-zdroje Online Plný text

NLK ProQuest Central od 2003-01-01 do Před 2 měsíci
Medline Complete (EBSCOhost) od 2012-09-01 do 2015-07-31
Nursing & Allied Health Database (ProQuest) od 2003-01-01 do Před 2 měsíci
Health & Medicine (ProQuest) od 2003-01-01 do Před 2 měsíci
Health Management Database (ProQuest) od 2003-01-01 do Před 2 měsíci
Public Health Database (ProQuest) od 2003-01-01 do Před 2 měsíci

Odkazy

PubMed 25770318
DOI 10.1016/j.nut.2014.08.010
Knihovny.cz E-zdroje

OBJECTIVES: There has been considerable interest in both clinical and preclinical research about the role of phytochemicals in the reduction of risk for cancer in humans. The aim of this study was to determine the antineoplastic effects of Chlorella pyrenoidosa in experimental breast cancer in vivo and in vitro. METHODS: In this experiment, the antineoplastic effects of C. pyrenoidosa in the chemoprevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis in female rats were evaluated. Chlorella powder was administered through diet at concentrations of 0.3% and 3%. The experiment was terminated 14 wk after carcinogen administration. At autopsy, mammary tumors were removed and prepared for histopathological and immunohistochemical analysis. In vitro cytotoxicity assay, parameters of apoptosis, and proliferation after chlorella treatment in human breast adenocarcinoma (MCF-7) cells were carried out. RESULTS: Basic parameters of experimental carcinogenesis, mechanism of action (biomarkers of apoptosis, proliferation, and angiogenesis), chosen metabolic variables, and side effects after long-term chlorella treatment in animals were assessed. Chlorella at higher concentration suppressed tumor frequency by 61% (P < 0.02) and lengthened tumor latency by 12.5 d (P < 0.02) in comparison with the controls. Immunohistochemical analysis of rat tumor cells showed caspase-7 expression increase by 73.5% (P < 0.001) and vascular endothelial growth factor receptor-2 expression decrease by 19% (P = 0.07) after chlorella treatment. In a parallel in vitro study, chlorella significantly decreased survival of MCF-7 cells in a dose-dependent manner. In chlorella-treated MCF-7 cells, a significant increase in cells having sub-G0/G1 DNA content and significant increase of early apoptotic and late apoptotic/necrotic cells after annexin V/PI staining assay were found. Decreases in mitochondrial membrane potential and increasing reactive oxygen species generation were observed in the chlorella-treated MCF-7 cells. CONCLUSIONS: This study is the first report on the antineoplastic effects of C. pyrenoidosa in experimental breast cancer in vivo and in vitro.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16000214
003      
CZ-PrNML
005      
20160126125456.0
007      
ta
008      
160108s2015 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.nut.2014.08.010 $2 doi
035    __
$a (PubMed)25770318
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Kubatka, Peter $u Department of Medical Biology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia. Electronic address: kubatka@jfmed.uniba.sk.
245    10
$a Antineoplastic effects of Chlorella pyrenoidosa in the breast cancer model / $c P. Kubatka, A. Kapinová, P. Kružliak, M. Kello, D. Výbohová, K. Kajo, M. Novák, M. Chripková, M. Adamkov, M. Péč, J. Mojžiš, B. Bojková, M. Kassayová, N. Stollárová, D. Dobrota,
520    9_
$a OBJECTIVES: There has been considerable interest in both clinical and preclinical research about the role of phytochemicals in the reduction of risk for cancer in humans. The aim of this study was to determine the antineoplastic effects of Chlorella pyrenoidosa in experimental breast cancer in vivo and in vitro. METHODS: In this experiment, the antineoplastic effects of C. pyrenoidosa in the chemoprevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis in female rats were evaluated. Chlorella powder was administered through diet at concentrations of 0.3% and 3%. The experiment was terminated 14 wk after carcinogen administration. At autopsy, mammary tumors were removed and prepared for histopathological and immunohistochemical analysis. In vitro cytotoxicity assay, parameters of apoptosis, and proliferation after chlorella treatment in human breast adenocarcinoma (MCF-7) cells were carried out. RESULTS: Basic parameters of experimental carcinogenesis, mechanism of action (biomarkers of apoptosis, proliferation, and angiogenesis), chosen metabolic variables, and side effects after long-term chlorella treatment in animals were assessed. Chlorella at higher concentration suppressed tumor frequency by 61% (P < 0.02) and lengthened tumor latency by 12.5 d (P < 0.02) in comparison with the controls. Immunohistochemical analysis of rat tumor cells showed caspase-7 expression increase by 73.5% (P < 0.001) and vascular endothelial growth factor receptor-2 expression decrease by 19% (P = 0.07) after chlorella treatment. In a parallel in vitro study, chlorella significantly decreased survival of MCF-7 cells in a dose-dependent manner. In chlorella-treated MCF-7 cells, a significant increase in cells having sub-G0/G1 DNA content and significant increase of early apoptotic and late apoptotic/necrotic cells after annexin V/PI staining assay were found. Decreases in mitochondrial membrane potential and increasing reactive oxygen species generation were observed in the chlorella-treated MCF-7 cells. CONCLUSIONS: This study is the first report on the antineoplastic effects of C. pyrenoidosa in experimental breast cancer in vivo and in vitro.
650    _2
$a zvířata $7 D000818
650    _2
$a annexin A5 $x metabolismus $7 D017304
650    _2
$a fytogenní protinádorové látky $x farmakologie $x terapeutické užití $7 D000972
650    _2
$a apoptóza $7 D017209
650    _2
$a nádory prsu $x farmakoterapie $x metabolismus $7 D001943
650    _2
$a kaspasa 7 $x metabolismus $7 D053179
650    _2
$a proliferace buněk $7 D049109
650    12
$a Chlorella $7 D002708
650    _2
$a dieta $7 D004032
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a lidé $7 D006801
650    _2
$a MFC-7 buňky $7 D061986
650    _2
$a membránový potenciál mitochondrií $x účinky léků $7 D053078
650    _2
$a mikrořasy $7 D058086
650    12
$a fytoterapie $7 D008517
650    _2
$a potkani Sprague-Dawley $7 D017207
650    _2
$a reaktivní formy kyslíku $x metabolismus $7 D017382
650    _2
$a vaskulární endoteliální růstový faktor A $x metabolismus $7 D042461
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Kapinová, Andrea $u Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia.
700    1_
$a Kružliak, Peter $u International Clinical Research Center, St. Anne's University Hospital and Masaryk University, Brno, Czech Republic.
700    1_
$a Kello, Martin $u Department of Pharmacology, Faculty of Medicine, P. J. Šafárik University, Košice, Slovakia.
700    1_
$a Výbohová, Desanka $u Department of Anatomy, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia.
700    1_
$a Kajo, Karol $u Department of Pathology, Slovak Medical University and St. Elisabeth Oncology Institute, Bratislava, Slovakia.
700    1_
$a Novák, Miroslav $u International Clinical Research Center, St. Anne's University Hospital and Masaryk University, Brno, Czech Republic.
700    1_
$a Chripková, Martina $u Department of Pharmacology, Faculty of Medicine, P. J. Šafárik University, Košice, Slovakia.
700    1_
$a Adamkov, Marián $u Department of Histology and Embryology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia. $7 gn_A_00001422
700    1_
$a Péč, Martin $u Department of Medical Biology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia.
700    1_
$a Mojžiš, Ján $u Department of Pharmacology, Faculty of Medicine, P. J. Šafárik University, Košice, Slovakia.
700    1_
$a Bojková, Bianka $u Department of Animal Physiology, Institute of Biological and Ecological Sciences, Faculty of Science, P. J. Šafárik University, Košice, Slovakia.
700    1_
$a Kassayová, Monika $u Department of Animal Physiology, Institute of Biological and Ecological Sciences, Faculty of Science, P. J. Šafárik University, Košice, Slovakia.
700    1_
$a Stollárová, Nadežda $u Department of Biology and Ecology, Faculty of Education, Catholic University in Ružomberok, Ružomberok, Slovakia.
700    1_
$a Dobrota, Dušan $u Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia.
773    0_
$w MED00003566 $t Nutrition (Burbank, Los Angeles County, Calif.) $x 1873-1244 $g Roč. 31, č. 4 (2015), s. 560-9
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25770318 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160108 $b ABA008
991    __
$a 20160126125619 $b ABA008
999    __
$a ok $b bmc $g 1102495 $s 924420
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 31 $c 4 $d 560-9 $e 20140906 $i 1873-1244 $m Nutrition $n Nutrition $x MED00003566
LZP    __
$a Pubmed-20160108

Najít záznam

Citační ukazatele

Možnosti archivace