-
Something wrong with this record ?
Probing single cells of purple sulfur bacteria with Raman spectroscopy: carotenoids and elemental sulfur
A. Oren, L. Mana, J. Jehlička,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
ProQuest Central
from 2015-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 2015-01-01 to 1 year ago
Public Health Database (ProQuest)
from 2015-01-01 to 1 year ago
PubMed
25682325
DOI
10.1093/femsle/fnv021
Knihovny.cz E-resources
- MeSH
- Single-Cell Analysis * methods MeSH
- Chromatiaceae chemistry ultrastructure MeSH
- Carotenoids analysis MeSH
- Spectrum Analysis, Raman methods MeSH
- Sulfur analysis MeSH
- Sulfides metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
We explored the use of Raman spectroscopy to simultaneously monitor the presence of different biomarkers (carotenoids, elemental sulfur) within single cells of the purple sulfur photosynthetic bacteria Allochromatium vinosum and A. warmingii. Raman microspectrometry using excitation at 532 nm allowed the detection of different carotenoids. Raman signals of elemental sulfur appeared soon after feeding starved cells with sulfide. Raman spectroscopy is thus a convenient and sensitive technique to qualitatively and semiquantitatively assess the presence of different compounds of interest within single bacterial cells.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16000287
- 003
- CZ-PrNML
- 005
- 20160125111623.0
- 007
- ta
- 008
- 160108s2015 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1093/femsle/fnv021 $2 doi
- 035 __
- $a (PubMed)25682325
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Oren, Aharon $u Department of Plant and Environmental Sciences, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus Givat Ram, Jerusalem 91904, Israel aharon.oren@mail.huji.ac.il.
- 245 10
- $a Probing single cells of purple sulfur bacteria with Raman spectroscopy: carotenoids and elemental sulfur / $c A. Oren, L. Mana, J. Jehlička,
- 520 9_
- $a We explored the use of Raman spectroscopy to simultaneously monitor the presence of different biomarkers (carotenoids, elemental sulfur) within single cells of the purple sulfur photosynthetic bacteria Allochromatium vinosum and A. warmingii. Raman microspectrometry using excitation at 532 nm allowed the detection of different carotenoids. Raman signals of elemental sulfur appeared soon after feeding starved cells with sulfide. Raman spectroscopy is thus a convenient and sensitive technique to qualitatively and semiquantitatively assess the presence of different compounds of interest within single bacterial cells.
- 650 _2
- $a karotenoidy $x analýza $7 D002338
- 650 _2
- $a Chromatiaceae $x chemie $x ultrastruktura $7 D002841
- 650 12
- $a analýza jednotlivých buněk $x metody $7 D059010
- 650 _2
- $a Ramanova spektroskopie $x metody $7 D013059
- 650 _2
- $a sulfidy $x metabolismus $7 D013440
- 650 _2
- $a síra $x analýza $7 D013455
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Mana, Lily $u Department of Plant and Environmental Sciences, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus Givat Ram, Jerusalem 91904, Israel.
- 700 1_
- $a Jehlička, Jan $u Institute of Geochemistry, Mineralogy and Mineral Resources, Charles University in Prague, 12843 Prague, Czech Republic.
- 773 0_
- $w MED00001792 $t FEMS microbiology letters $x 1574-6968 $g Roč. 362, č. 6 (2015)
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/25682325 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20160108 $b ABA008
- 991 __
- $a 20160125111745 $b ABA008
- 999 __
- $a ok $b bmc $g 1102568 $s 924493
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 362 $c 6 $e 20150214 $i 1574-6968 $m FEMS microbiology letters $n FEMS Microbiol Lett $x MED00001792
- LZP __
- $a Pubmed-20160108
