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Novel biophysical determination of miRNAs related to prostate and head and neck cancers
K. Hudcova, L. Trnkova, I. Kejnovska, M. Vorlickova, J. Gumulec, R. Kizek, M. Masarik,
Language English Country Germany
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NT14337
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
ProQuest Central
from 1997-01-01 to 2019-01-31
Medline Complete (EBSCOhost)
from 1996-11-01 to 1 year ago
Health & Medicine (ProQuest)
from 1997-01-01 to 2019-01-31
- MeSH
- Circular Dichroism methods MeSH
- Electrochemistry methods MeSH
- Photoelectron Spectroscopy methods MeSH
- Humans MeSH
- MicroRNAs blood MeSH
- Biomarkers, Tumor blood MeSH
- Head and Neck Neoplasms blood MeSH
- Prostatic Neoplasms blood MeSH
- Case-Control Studies MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
In this study we have chosen a new approach and characterized three miRNAs (miR-23a, miR-34a and miR-320a) related to prostate cancer and head and neck cancer by spectral (circular dichroic and UV-absorption spectra) and electrochemical (voltammetry at graphite and mercury electrodes) methods. The spectral and voltammetric results, reflecting different nucleotide sequences of miRNAs, were complemented by the results of DNAs(U) having the same oligonucleotide sequences as miRNAs. The effect of the substitution of ribose for deoxyribose was shown and structural diversity was confirmed. The stability of RNA and DNA(U) was studied using CD and UV-absorption spectroscopy and melting points were calculated. MiRNA-320a with the highest content of guanine provided the highest melting point. With respect to the rapid progress of miRNA electrochemical sensors, our results will be useful for the research and development of sensitive, portable and time-efficient miRNA sensors, which will be able to diagnose cancer and other diseases.
References provided by Crossref.org
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- $a In this study we have chosen a new approach and characterized three miRNAs (miR-23a, miR-34a and miR-320a) related to prostate cancer and head and neck cancer by spectral (circular dichroic and UV-absorption spectra) and electrochemical (voltammetry at graphite and mercury electrodes) methods. The spectral and voltammetric results, reflecting different nucleotide sequences of miRNAs, were complemented by the results of DNAs(U) having the same oligonucleotide sequences as miRNAs. The effect of the substitution of ribose for deoxyribose was shown and structural diversity was confirmed. The stability of RNA and DNA(U) was studied using CD and UV-absorption spectroscopy and melting points were calculated. MiRNA-320a with the highest content of guanine provided the highest melting point. With respect to the rapid progress of miRNA electrochemical sensors, our results will be useful for the research and development of sensitive, portable and time-efficient miRNA sensors, which will be able to diagnose cancer and other diseases.
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