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Detection of biomarkers for inflammatory diseases by an electrochemical immunoassay: the case of neopterin

S. Centi, S. Tombelli, M. Puntoni, C. Domenici, M. Franek, I. Palchetti,

. 2015 ; 134 (-) : 48-53. [pub] 20141104

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16000349

An electrochemical immunoassay for neopterin was developed using recently produced specific antibodies immobilized to protein A-coated magnetic beads in combination with differential pulse voltammetry and screen-printed array of electrodes. Neopterin-alkaline phosphatase conjugate was used as label in a competitive assay format. Multiplexed analysis of neopterin was demonstrated by replacing the traditional ELISA with electrochemical detection and the traditional plastic wells with screen-printed array of electrodes. The optimized electrochemical method, based on polyclonal antibodies, reached a limit of detection of 0.008 ng/mL with an average RSD %=10. Serum samples collected from patients with sepsis, healthy volunteers and other patients without a confirmed clinical diagnosis were also analyzed. The obtained results, compared with those of a commercial ELISA kit, had a significant correlation, showing the possibility to distinguish among the serum samples from ill or healthy subjects.

Citace poskytuje Crossref.org

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$a Domenici, Claudio $u Istituto di Fisiologia Clinica, Consiglio Nazionale delle Ricerche, Via G. Moruzzi 1, 56124 Pisa, Italy.
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$a Palchetti, Ilaria $u Università degli Studi di Firenze, Dipartimento di Chimica, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy; Istituto di Biochimica delle Proteine, Via P. Castellino 111, 80131 Napoli, Italy. Electronic address: ilaria.palchetti@unifi.it.
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