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Differential impact of stress on hypothalamic-pituitary-adrenal axis: gene expression changes in Lewis and Fisher rats
P. Ergang, M. Vodička, M. Soták, P. Klusoňová, M. Behuliak, L. Řeháková, P. Zach, J. Pácha,
Language English Country England, Great Britain
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- 11-beta-Hydroxysteroid Dehydrogenase Type 1 genetics metabolism MeSH
- Amygdala metabolism MeSH
- Arginine Vasopressin genetics metabolism MeSH
- Hippocampus metabolism MeSH
- Corticotropin-Releasing Hormone genetics metabolism MeSH
- Pituitary Gland metabolism MeSH
- Pituitary Adenylate Cyclase-Activating Polypeptide genetics metabolism MeSH
- Rats MeSH
- Adrenal Cortex metabolism MeSH
- RNA, Messenger metabolism MeSH
- Brain metabolism MeSH
- Paraventricular Hypothalamic Nucleus metabolism MeSH
- Oxytocin genetics metabolism MeSH
- Rats, Inbred F344 MeSH
- Rats, Inbred Lew MeSH
- Prefrontal Cortex metabolism MeSH
- Stress, Psychological genetics metabolism MeSH
- Receptors, Glucocorticoid genetics metabolism MeSH
- Gene Expression Profiling MeSH
- Pituitary-Adrenal System metabolism MeSH
- Hypothalamo-Hypophyseal System metabolism MeSH
- Urocortins genetics metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
The aim of the present work was to study the influence of variable stress on the expression of 11β-hydroxysteroid dehydrogenase type 1 (11HSD1) and the neuropeptides corticotropin-releasing hormone (CRH), urocortins 2 and 3(UCN2, UCN3), arginine vasopressin (AVP), oxytocin (OXT) and adenylate cyclase-activating polypeptide (PACAP) in two inbred rat strains: stress hypo-responsive Lewis (LEW) and hyper-responsive Fisher 344 (F344) rats. We found site-specific and strain-dependent differences in the basal and stress-stimulated expression of 11HSD1, CRH, UCN2, UCN3 and PACAP. In LEW rats, stress upregulated 11HSD1 in the prefrontal cortex and lateral amygdala, whereas in F344 rats 11HSD1 was upregulated in the central amygdala and hippocampal CA2 and ventral but not dorsal CA1 region; no effect was observed in the paraventricular nucleus, pituitary gland and adrenal cortex of both strains. The expression of glucocorticoid receptors did not parallel the upregulation of 11HSD1. Stress also stimulated the expression of paraventricular OXT, CRH, UCN3 and PACAP in both strains but amygdalar CRH only in LEW and UCN2/UCN3 in F344 rats, respectively. The upregulation of PACAP and CRH was paralleled only by increased expression of PACAP receptor PAC1 but not CRH receptor type 1. These observations provide evidence that inbred F344 and LEW rats exhibit not only the well-known phenotypic differences in the activity of the HPA axis but also strain- and stress-dependent differences in the expression of genes encoding 11HSD1 and neuropeptides associated with the HPA axis activity. Moreover, the differences in 11HSD1 expression suggest different local concentration of corticosterone and access to GR in canonical and noncanonical structures of the HPA axis.
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