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Lateral resolution in NALDI MSI: back to the future
L. Krasny, O. Benada, M. Strnadova, K. Lemr, V. Havlicek,
Language English Country Germany
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
NLK
ProQuest Central
from 2011-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2003-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 2011-01-01 to 1 year ago
- MeSH
- Diagnostic Imaging MeSH
- Lasers MeSH
- Kidney chemistry MeSH
- Mice MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization instrumentation methods MeSH
- Tandem Mass Spectrometry instrumentation methods MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
Nanostructure-assisted laser desorption/ionization (NALDI) has been recognized as a powerful matrix-free mass spectrometry tool ideal for imaging of small molecules. In this report, the NALDI approach was compared with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry in terms of sensitivity, reproducibility, and lateral resolution, which can be achieved in mass spectrometry imaging (MSI) experiments using a Nd:YAG laser. Scanning electron microscopy was used for surface topology analysis and evaluation of a putative surface-enhanced sensitivity effect, which was observed upon reduction of the laser focus. NALDI was identified as a more reproducible technique lacking MSI artifacts arising from distant tissue removal known from MALDI oversampling.
References provided by Crossref.org
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