• Something wrong with this record ?

Predilection sites for Toxoplasma gondii in sheep tissues revealed by magnetic capture and real-time PCR detection

J. Juránková, W. Basso, H. Neumayerová, A. Frencová, V. Baláž, P. Deplazes, B. Koudela,

. 2015 ; 52 (-) : 150-3. [pub] 20150707

Language English Country England, Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc16009978

Undercooked lamb and mutton are common sources of Toxoplasma gondii infection for humans. A sequence specific magnetic capture technique in combination with quantitative real-time PCR targeting the 529 bp repeat element of T. gondii was used for estimation of the parasite burdens in various sheep tissues (n = 6) three months after peroral experimental inoculation with 10,000 T. gondii oocysts. Brain was the most frequently affected organ (positive in all 6 sheep) and showed the highest estimated parasite loads (0.5-30,913 parasites/g tissue). Lung samples were positive in three sheep, with load estimates of 36.3 to <1 parasite/g tissue. Heart tissue was positive in three sheep and kidney only in one animal with low parasite loads (<1 parasite/g tissue). Only few skeletal muscle samples in 2 animals showed positive results, with very low parasite burdens, while samples from further internal organs (i.e. liver and spleen) were negative in all animals. This study identified the brain as the most important predilection site and therefore the most appropriate tissue for T. gondii detection.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16009978
003      
CZ-PrNML
005      
20160413114723.0
007      
ta
008      
160408s2015 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.fm.2015.07.005 $2 doi
024    7_
$a 10.1016/j.fm.2015.07.005 $2 doi
035    __
$a (PubMed)26338129
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Juránková, Jana $u Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic. Electronic address: yanniska@seznam.cz.
245    10
$a Predilection sites for Toxoplasma gondii in sheep tissues revealed by magnetic capture and real-time PCR detection / $c J. Juránková, W. Basso, H. Neumayerová, A. Frencová, V. Baláž, P. Deplazes, B. Koudela,
520    9_
$a Undercooked lamb and mutton are common sources of Toxoplasma gondii infection for humans. A sequence specific magnetic capture technique in combination with quantitative real-time PCR targeting the 529 bp repeat element of T. gondii was used for estimation of the parasite burdens in various sheep tissues (n = 6) three months after peroral experimental inoculation with 10,000 T. gondii oocysts. Brain was the most frequently affected organ (positive in all 6 sheep) and showed the highest estimated parasite loads (0.5-30,913 parasites/g tissue). Lung samples were positive in three sheep, with load estimates of 36.3 to <1 parasite/g tissue. Heart tissue was positive in three sheep and kidney only in one animal with low parasite loads (<1 parasite/g tissue). Only few skeletal muscle samples in 2 animals showed positive results, with very low parasite burdens, while samples from further internal organs (i.e. liver and spleen) were negative in all animals. This study identified the brain as the most important predilection site and therefore the most appropriate tissue for T. gondii detection.
650    _2
$a anatomické struktury zvířat $x parazitologie $7 D000825
650    _2
$a zvířata $7 D000818
650    _2
$a magnetismus $7 D008280
650    _2
$a kvantitativní polymerázová řetězová reakce $x přístrojové vybavení $x metody $7 D060888
650    _2
$a ovce $7 D012756
650    _2
$a Toxoplasma $x genetika $x izolace a purifikace $7 D014122
650    _2
$a toxoplazmóza zvířat $x parazitologie $7 D014124
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Basso, Walter $u Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zurich, Switzerland.
700    1_
$a Neumayerová, Helena $u Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic.
700    1_
$a Frencová, Anita $u Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic.
700    1_
$a Baláž, Vojtech $u Department of Biology and Wildlife Diseases, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic.
700    1_
$a Deplazes, Peter $u Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zurich, Switzerland.
700    1_
$a Koudela, Břetislav $u Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic; Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic.
773    0_
$w MED00001834 $t Food microbiology $x 1095-9998 $g Roč. 52, č. - (2015), s. 150-3
856    41
$u https://pubmed.ncbi.nlm.nih.gov/26338129 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160408 $b ABA008
991    __
$a 20160413114807 $b ABA008
999    __
$a ok $b bmc $g 1113407 $s 934346
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 52 $c - $d 150-3 $e 20150707 $i 1095-9998 $m Food microbiology $n Food Microbiol $x MED00001834
LZP    __
$a Pubmed-20160408

Find record

Citation metrics

Archiving options