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A mass spectrometric-derived cell surface protein atlas
D. Bausch-Fluck, A. Hofmann, T. Bock, AP. Frei, F. Cerciello, A. Jacobs, H. Moest, U. Omasits, RL. Gundry, C. Yoon, R. Schiess, A. Schmidt, P. Mirkowska, A. Härtlová, JE. Van Eyk, JP. Bourquin, R. Aebersold, KR. Boheler, P. Zandstra, B. Wollscheid,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2006
Free Medical Journals
od 2006
Public Library of Science (PLoS)
od 2006
PubMed Central
od 2006
Europe PubMed Central
od 2006
ProQuest Central
od 2006-12-01
Open Access Digital Library
od 2006-10-01
Open Access Digital Library
od 2006-01-01
Open Access Digital Library
od 2006-01-01
Medline Complete (EBSCOhost)
od 2008-01-01
Nursing & Allied Health Database (ProQuest)
od 2006-12-01
Health & Medicine (ProQuest)
od 2006-12-01
Public Health Database (ProQuest)
od 2006-12-01
ROAD: Directory of Open Access Scholarly Resources
od 2006
- MeSH
- buněčné linie MeSH
- databáze proteinů MeSH
- hmotnostní spektrometrie metody MeSH
- lidé MeSH
- membránové proteiny chemie MeSH
- myši MeSH
- proteomika metody MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
Cell surface proteins are major targets of biomedical research due to their utility as cellular markers and their extracellular accessibility for pharmacological intervention. However, information about the cell surface protein repertoire (the surfaceome) of individual cells is only sparsely available. Here, we applied the Cell Surface Capture (CSC) technology to 41 human and 31 mouse cell types to generate a mass-spectrometry derived Cell Surface Protein Atlas (CSPA) providing cellular surfaceome snapshots at high resolution. The CSPA is presented in form of an easy-to-navigate interactive database, a downloadable data matrix and with tools for targeted surfaceome rediscovery (http://wlab.ethz.ch/cspa). The cellular surfaceome snapshots of different cell types, including cancer cells, resulted in a combined dataset of 1492 human and 1296 mouse cell surface glycoproteins, providing experimental evidence for their cell surface expression on different cell types, including 136 G-protein coupled receptors and 75 membrane receptor tyrosine-protein kinases. Integrated analysis of the CSPA reveals that the concerted biological function of individual cell types is mainly guided by quantitative rather than qualitative surfaceome differences. The CSPA will be useful for the evaluation of drug targets, for the improved classification of cell types and for a better understanding of the surfaceome and its concerted biological functions in complex signaling microenvironments.
Center for Systems Physiology and Metabolic Diseases Zurich Switzerland
Department of Biochemistry Medical College of Wisconsin Wisconsin Milwaukee United States of America
Department of Health Sciences and Technology BMPP ETH Zurich Zurich Switzerland
Faculty of Science University of Zurich Zurich Switzerland
Institute for Biomaterials and Biomedical Engineering University of Toronto Toronto Canada
Institute of Molecular Systems Biology ETH Zurich Zurich Switzerland
Laboratory of Molecular Oncology University Hospital Zurich Zurich Switzerland
Oncology Research Laboratory University Children Hospital Zurich Zurich Switzerland
SCRMC LKS Faculty of Medicine Hong Kong University Hong Kong Hong Kong SAR
Citace poskytuje Crossref.org
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