• Je něco špatně v tomto záznamu ?

Interactions of hyaluronic Acid with the skin and implications for the dermal delivery of biomacromolecules

M. Witting, A. Boreham, R. Brodwolf, K. Vávrová, U. Alexiev, W. Friess, S. Hedtrich,

. 2015 ; 12 (5) : 1391-401. [pub] 20150422

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc16010339

Hyaluronic acid (HA) hydrogels are interesting delivery systems for topical applications. Besides moisturizing the skin and improving wound healing, HA facilitates topical drug absorption and is highly compatible with labile biomacromolecules. Hence, in this study we investigated the influence of HA hydrogels with different molecular weights (5 kDa, 100 kDa, 1 MDa) on the skin absorption of the model protein bovine serum albumin (BSA) using fluorescence lifetime imaging microscopy (FLIM). To elucidate the interactions of HA with the stratum corneum and the skin absorption of HA itself, we combined FLIM and Fourier-transform infrared (FTIR) spectroscopy. Our results revealed distinct formulation and skin-dependent effects. In barrier deficient (tape-stripped) skin, BSA alone penetrated into dermal layers. When BSA and HA were applied together, however, penetration was restricted to the epidermis. In normal skin, penetration enhancement of BSA into the epidermis was observed when applying low molecular weight HA (5 kDa). Fluorescence resonance energy transfer analysis indicated close interactions between HA and BSA under these conditions. FTIR spectroscopic analysis of HA interactions with stratum corneum constituents showed an α-helix to β-sheet interconversion of keratin in the stratum corneum, increased skin hydration, and intense interactions between 100 kDa HA and the skin lipids resulting in a more disordered arrangement of the latter. In conclusion, HA hydrogels restricted the delivery of biomacromolecules to the stratum corneum and viable epidermis in barrier deficient skin, and therefore seem to be potential topical drug vehicles. In contrast, HA acted as an enhancer for delivery in normal skin, probably mediated by a combination of cotransport, increased skin hydration, and modifications of the stratum corneum properties.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16010339
003      
CZ-PrNML
005      
20160415122107.0
007      
ta
008      
160408s2015 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1021/mp500676e $2 doi
024    7_
$a 10.1021/mp500676e $2 doi
035    __
$a (PubMed)25871518
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Witting, Madeleine $u †Department of Pharmaceutical Sciences, Ludwig-Maximilians-Universität, Munich, Germany.
245    10
$a Interactions of hyaluronic Acid with the skin and implications for the dermal delivery of biomacromolecules / $c M. Witting, A. Boreham, R. Brodwolf, K. Vávrová, U. Alexiev, W. Friess, S. Hedtrich,
520    9_
$a Hyaluronic acid (HA) hydrogels are interesting delivery systems for topical applications. Besides moisturizing the skin and improving wound healing, HA facilitates topical drug absorption and is highly compatible with labile biomacromolecules. Hence, in this study we investigated the influence of HA hydrogels with different molecular weights (5 kDa, 100 kDa, 1 MDa) on the skin absorption of the model protein bovine serum albumin (BSA) using fluorescence lifetime imaging microscopy (FLIM). To elucidate the interactions of HA with the stratum corneum and the skin absorption of HA itself, we combined FLIM and Fourier-transform infrared (FTIR) spectroscopy. Our results revealed distinct formulation and skin-dependent effects. In barrier deficient (tape-stripped) skin, BSA alone penetrated into dermal layers. When BSA and HA were applied together, however, penetration was restricted to the epidermis. In normal skin, penetration enhancement of BSA into the epidermis was observed when applying low molecular weight HA (5 kDa). Fluorescence resonance energy transfer analysis indicated close interactions between HA and BSA under these conditions. FTIR spectroscopic analysis of HA interactions with stratum corneum constituents showed an α-helix to β-sheet interconversion of keratin in the stratum corneum, increased skin hydration, and intense interactions between 100 kDa HA and the skin lipids resulting in a more disordered arrangement of the latter. In conclusion, HA hydrogels restricted the delivery of biomacromolecules to the stratum corneum and viable epidermis in barrier deficient skin, and therefore seem to be potential topical drug vehicles. In contrast, HA acted as an enhancer for delivery in normal skin, probably mediated by a combination of cotransport, increased skin hydration, and modifications of the stratum corneum properties.
650    _2
$a aplikace kožní $7 D000279
650    _2
$a zvířata $7 D000818
650    _2
$a skot $7 D002417
650    _2
$a kyselina hyaluronová $x chemie $7 D006820
650    _2
$a techniky in vitro $7 D066298
650    _2
$a sérový albumin hovězí $x chemie $x metabolismus $7 D012710
650    _2
$a kůže $x metabolismus $7 D012867
650    _2
$a kožní absorpce $7 D012869
650    _2
$a spektroskopie infračervená s Fourierovou transformací $7 D017550
650    _2
$a prasata $7 D013552
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Boreham, Alexander $u ‡Department of Physics, Institute of Experimental Physics, Freie Universität Berlin, Berlin, Germany.
700    1_
$a Brodwolf, Robert $u ‡Department of Physics, Institute of Experimental Physics, Freie Universität Berlin, Berlin, Germany.
700    1_
$a Vávrová, Kateřina $u §Department of Inorganic and Organic Chemistry, Faculty of Pharmacy, Charles University in Prague, Hradec Kralove, Czech Republic.
700    1_
$a Alexiev, Ulrike $u ‡Department of Physics, Institute of Experimental Physics, Freie Universität Berlin, Berlin, Germany. $7 gn_A_00004033
700    1_
$a Friess, Wolfgang $u †Department of Pharmaceutical Sciences, Ludwig-Maximilians-Universität, Munich, Germany.
700    1_
$a Hedtrich, Sarah $u †Department of Pharmaceutical Sciences, Ludwig-Maximilians-Universität, Munich, Germany. ∥Institute for Pharmaceutical Sciences, Freie Universität Berlin, Berlin, Germany.
773    0_
$w MED00008279 $t Molecular pharmaceutics $x 1543-8392 $g Roč. 12, č. 5 (2015), s. 1391-401
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25871518 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160408 $b ABA008
991    __
$a 20160415122152 $b ABA008
999    __
$a ok $b bmc $g 1113768 $s 934707
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 12 $c 5 $d 1391-401 $e 20150422 $i 1543-8392 $m Molecular pharmaceutics $n Mol Pharm $x MED00008279
LZP    __
$a Pubmed-20160408

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace